摘要
通过诱导胚状体建立组织培养再生系统，研究了球根海棠“金正日”的离体培养。结果表明，外植体经HgCl2溶液表面消毒后，接种到MS＋6-BA0．2mg／L＋NAA 1mg/L培养基上诱导愈伤组织，15d后转接到1／2MS＋6-BA0．5mg/L＋NAA 0．1mg/L培养基上，诱导胚状体，诱导率90％以上。胚状体在MS＋6-BA0．3mg／L＋NAA0．03mg／L培养基上以丛生芽形式增殖，增殖系数达4～8倍。试管苗生根可采用MS＋IBA0．1mg/L、蔗糖2％培养基。生根率100％。
The tissue culture and regenerate system of Jinzhengri were established through inducting embryo. The explants with surface sterilization of HgCl2 were inoculated on the MS medium containing 6-BA 0.2 mg/L＋ NAA 1 mg/L for inducting callus. After 15 days, the calli were removed on the medium with 1/2 MS ＋ 6-BA 0.5 mg/L＋ NAA 0.1 mg/L for inducting the embryo, with the induction rate of above 90%. The embryos were propagated in form of cespitose buds on the medium with MS ＋ 6-BA 0.3 mg/L ＋ NAA 0.03 mg/L, with the propagation coefficient reaching 4 to 8 times. The root induction rate of test-tube seedlings was 100% on the medium with MS＋ IBA 0.1 mg/L ＋ 2% saccharose.
出处
《安徽农业科学》
CAS
北大核心
2007年第10期2906-2906,3035,共2页
Journal of Anhui Agricultural Sciences
关键词
球根海棠
金正日
胚状体
组织培养
Begonia tuberhybridu
Jinzhengri
Embryo
Tissue culture
