摘要
对采自陕西、山西、甘肃、河南、河北、山东各省的泡桐丛枝病材料,利用巢式扩增,均得到16S rDNA基因片段约1.2kb;扩增得到植原体延伸因子(EF-Tu)tuf基因,长度约为850bp.。通过将16S rDNA基因片段和延伸因子(EF-Tu)tuf基因序列与已知植原体16Sr各组成员进行同源性比较,确定我国大陆泡桐主栽区陕西、山西、甘肃、河南、河北、山东各省与已经报道的台湾省泡桐丛枝植原体基本一致,均为同一个种,没有株系的分化,全部归属于植原体16SrI-D组,从而确定了其分类地位。
A 1.2kb of 16S rDNA length frgement is obtained by nest polymerase chain reaction from paulownia witches phytoplasma samples which were collected from Shaanxi, Shanxi, Gansu, Henan, Hebei, Shandong Province, and also a 850bp DNA fragement of tuf （ EF- Tu） gene was amplified from above samples using primers （fTufu/rTufu） . Homologic analysis shows that it is the same of 16S rDNA nucleotide sequences of PaWB-Shaanxi, PaWB-Taiwan and that of PaWB from Shanxi, Gansu, Henan, Hebei, Shandong Province. All species of PaWB phytoplasma, from different regions, are believed to be a 16Sr I-D group of phytoplasma. Therefore, we can confirm classified position of PaWB phytoplasma from China.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第2期291-295,共5页
Microbiology China
基金
教育部长江学者和创新团队发展计划资助(No200558)
