摘要
以穿龙薯蓣种子为外植体诱导出了愈伤组织,最佳培养基为MS+6-BA3.0mg/L+NAA2.0mg/L,最高出愈率为18.9%;诱导不定芽的最佳培养基为MS+6-BA2.0mg/L+NAA2.0mg/L,最高诱导率可达75.5%;最佳壮苗培养基为1/2MS+6-BA 1.0mg/L+KT1.0m g/L;根诱导的最佳培养基为1/2MS+6-BA0.5mg/L+NAA0.5mg/L,诱导率最高达86.2%;最佳的炼苗基质为腐质土∶蛭石∶河沙=1∶1∶1,成活率最高达84.8%。
Callus was induced using seeds as explant on Dioscorea nipponica Makino, the best culture media is MS＋6-BA3.0 mg/L＋NAA2.0 mg/L and the highest frequency is 18.9%. The gemma can be induced when the callus was transferred into the culture medium with MS＋6-BA2.0mg/L＋NAA2.0mg/L and the highest inducement frequency can be reach 75.5%. The buds grow strongly on the culture medium with 1/2MS＋6-BA1.0mg/L＋KT1.0mg]L and radicate easily on the culture medium with I/2MS ＋6-BA 0.Smg/L ＋NAA 0.5mg/L. Radication frequency can be reach 86.2%.The transplanting medium in rotten quality soil, vermiculite and fiver sand by 1 to 1 to 1, is useful,84,.8% seedlings can survive if the technique is fine.
出处
《特产研究》
2007年第1期27-30,共4页
Special Wild Economic Animal and Plant Research
关键词
穿龙薯蓣
种子
组织培养
愈伤组织
Dioscorea nipponica Makino.
Seed
Tissue culture
Callus
