摘要
目的观察年龄相关人类卵泡液活性氧(ROS)水平和颗粒细胞线粒体活性变化,探讨卵母细胞年龄相关改变的发生机制。方法运用DCFH-DA和线粒体活性特异性染料DioC6,采用增强化学发光法和流式细胞仪技术,分别检测了53例体外受精患者卵泡液ROS水平和黄素化颗粒细胞线粒体活性。其中小于等于37岁组34例,大于37岁组19例。结果卵泡液内ROS水平和颗粒细胞线粒体活性与母体年龄相关,年长妇女卵泡液ROS水平较年轻组高,而颗粒细胞线粒体活性降低。结论年长妇女颗粒细胞线粒体活性下降和卵泡液ROS的升高可能是卵母细胞年龄相关改变的原因之一。
Objective ： To examine the age - related change of reactive oxygen species （ROS） level in follicular fluid and mitochondrial activity in the granulocyte of women undergoing in vitro fertilization （IVF） and identify its relation to age - related oocyte change. Methods ： ROS levels in follicular fluid were measured by the chemiluminescence method. Mitochondrial activity of granulocyte were measured by flow cytometer. 53 samples were divided into 2 group according to age,34 cases were in young group（ 〈 37 years ）, 19 cases were in older group （≥37 years）. Results： The level of ROS in follicular fluid in young group was lower than that in older group, while mitochondria activity of granulocyte was opposite. Conclusion ： Reproductive ageing is accompanied by a change in the mitochondria activity of granulocyte that could impair ROS scavenging efficiency in the follicular environment.
出处
《中国优生与遗传杂志》
2007年第3期85-86,共2页
Chinese Journal of Birth Health & Heredity
关键词
颗粒细胞
活性氧
女性生殖
年龄
Reactive oxygen species
Granulocyte
Female reprodution
Age
