摘要
目的:探讨从弯斑蛸的肌肉、生殖腺、消化腺中提取的3种多糖OGl、OG2和OG3对小鼠免疫活性的影响。方法:MTT比色法测定脾细胞的增殖和免疫器官重量法测定免疫器官指数。结果:OGl和OG2对正常小鼠脾细胞和免疫抑制小鼠脾细胞增殖均有促进作用,对于免疫抑制小鼠脾细胞增殖作用更为显著,与ConA合用均无协同作用,OG3对两种脾细胞均出现抑制作用;3种多糖对正常小鼠和CTX所致免疫低下小鼠的免疫器官都有较明显的提高作用,可对抗CTX所致的脾萎缩作用,对胸腺指数提高更为明显。结论:OGl和OG2可提高正常和免疫低下小鼠的免疫功能,对于后者效果更好;OG3体外对脾细胞有抑制作用,体内试验有较好提高免疫效果。
Objective：To study the immunoloregulation effect of three polysaccharides OG1, OG2 and OG3 extracted from Octopus dollfusi muscle, gonad, digestive gland, respectively. Methods ： Spleen cell proliferation was measured by MTT assay and index of immune organs were weighed and calculated. Results ： OG1 and OG2 could increase the proliferation of mouse spleen cell of normal mice, and significantly increased the proliferation of the spleen of immunosuppression mice caused by sccyclophosphamide, while showed no cooperation with ConA; OG3 appeared suppression for the two spleens. The three polysaccharides could increase index of immune organs of normal mice and remarkably increased the indexof immunosuppression mice caused by sccyclophosphamide;while showed obvious function on thymus index. Conclusion ：These results suggest that OG1 and OG2 have enhancement immunity for normal and immunosuppression mice,and have better effect for the latter;OG3 has suppression proliferation the spleen cell in vitro,however it has better effect for increase index of immune organs in vivo.
出处
《中药材》
CAS
CSCD
北大核心
2007年第2期188-191,共4页
Journal of Chinese Medicinal Materials
基金
广东省科技厅项目(No.2003C32805)
关键词
弯斑蛸
多糖
小鼠
脾细胞
免疫器官
Octopus doUfusi
Polysaccharide
Mouse
Spleen cell
Immune organ
