摘要
目的:检测内皮素-1(ET-1)及其受体在肺动脉高压大鼠肺组织中的表达,以探讨左向右分流肺动脉高压的发病机理。方法:选择雄性Wistar大鼠34只,随机分为实验Ⅰ组(n=15)、Ⅱ组(n=9)、对照组(n=10)。实验组大鼠用套管法建立右侧颈部左向右分流模型;术后4周,实验Ⅱ组大鼠给予BQ123〔cyclo-propane(Trp-Asp-Pro-Val-Leu)〕,连用12周;术后16周测取大鼠肺动脉收缩压;留取肺组织,以放射免疫法测定肺组织中ET-1含量;RT-PCR法检测肺组织中PPET-1mRNA、ETA及ETB受体mRNA的表达。结果:①与实验Ⅱ组及对照组相比,实验Ⅰ组大鼠肺动脉压及肺组织ET-1含量明显升高(P<0.05,P<0.001,P<0.01,P<0.001),PPET-1 mRNA和ETA受体mRNA的表达明显增强(P<0.05,P<0.001),ETB受体mRNA的表达明显降低(P<0.01,P<0.001);②与对照组相比,实验Ⅱ组大鼠肺组织PPET-1mRNA增强(P<0.05),ETB受体mRNA的表达下降(P<0.05)。结论:不同类型的ET-1受体在左向右分流肺动脉压变化中的作用不同。
Objective： To investigate the mechanism of pulmonary hypertension, the effect of ET-1 receptors in pulmonary hypertension due to left to right shunt was studied. Methods： Thirty-four 150-200g male Wistar rats were randomly selected and divided into three groups： experiment group Ⅰ （n = 15）, experiment group Ⅱ （n = 9） and control group （ n = 10）. A cervical left to right shunt pulmonary hypertension model was established by the cuff technique in the experiment groups. After four weeks, the rats of experiment group Ⅱ underwent a 12-week intravenous administration of BQ123 [ cyclopropane （Trp-Asp-Pro-Val-Leu） ]. The systolic pulmonary artery pressures （sPAPs）, ET-1 concentrations and the expressions of the prepro ET-1 （PPET-1） mRNA, ETA receptor mRNA and ETB receptor mRNA of pulmonary tissues in the three groups were measured. Results： The sPAP and ET-1 concentrations were significantly higher in the experiment groups than in the control group （ P 〈 0.01 ）, and they were higher in experiment group Ⅰ than in experiment groupⅡ . The mRNA expression of ET-1 and ETA receptors were distinctly elevated in the experiment groups （ P 〈 0.01）, and they were higher in experiment group Ⅰ than in experiment group Ⅱ （P 〈 0.05）. ETB receptors changed inversely. Condusions： The above results indicate that different ET receptors may exhibit different effects on pulmonary hypertension progression due to left to right shunt.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第2期142-145,共4页
Journal of Shandong University:Health Sciences
基金
山东省卫生厅科研基金资助课题(2001CA1CJB2)
