摘要
目的建立测定头孢他啶血药浓度的方法。方法高效液相色谱法采用Kromasil C18色谱柱（250mm×4．6mm，5μm），以甲醇-磷酸二氢钾溶液（15：85）为流动相，流速1．0ml／min，检测波长254nm。结果头孢他啶吼浆浓度在0．5～200．0mg·L^-1范围内线性笑系良好（r=0．9999）。低、中、高3个浓度的方法回收率为94．91％～105．95％，RSD〈12％；提取回收率为89．50％～92．94％，RSD〈5％。结论本方法简便、准确、精密度好，可用于临床药动学研究。
Aim To establish a method for the determination of ceftazidime concentration in plasma. Method The HPLC separation was performed on Kromasil C18 column （250mm × 4.6mm, 5μm）. The mobile phase was methanol-potassium dihydrogen phosphate （ 15：85 ） at a rate of 1.0mlYmin. The detection wavelength was 254nm. Results The linear range was 0.5 - 200.0mg·L^-1 （ r = 0. 999 9 ）. The method recovery of ceftazidime was 94. 91% - 105.95% （ RSDs lower than 12% ） and the extraction recovery of ceftazidime was 89.50% - 92.94% （ RSDs lower than 5% ）. Conclusion The method appeared to be simple, accurate and precise. It is suitable for clinical pharmacokinetic study.
出处
《解放军药学学报》
CAS
2007年第1期41-43,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
全军医学科学技术研究"十一五"计划科技攻关课题资助项目
No.06G023
