摘要
目的:考察绵茵陈提取液的静态吸附工艺的影响因素,以确定其大孔吸附树脂工艺路线和参数。方法:以吸光度和绿原酸的比吸附量为指标,静态吸附法考察9种树脂的吸附效果和绵茵陈提取液在F树脂的吸附时间曲线;采用析因实验设计,静态吸附法考察上柱药液浓度和pH对绿原酸吸附的影响;以绵茵陈提取液和洗脱液的HPLC指纹图谱为指标,考察其精制效果。结果:不同树脂对绵茵陈提取液中成分的吸附效果有差异;绵茵陈提取液在F树脂的吸附平衡时间为6 h;上柱药液浓度和pH是影响吸附的重要因素。验证实验表明该工艺在有效保留HPLC指纹图谱成分的同时,使提取液的得膏率降低到2.5%。结论:该结果可为绵茵陈提取液的大孔树脂精制工艺路线的确定提供参考。
AIM ： To study the influencing factors of static adsorpting process of extract of Herba Artemisiae scopariae with macroporous resin, and determine the refinement process. METHODS： The absorbances and the adsorption quantity of caffeotannic acid were used as indexes, the adsorption effect of 9 kinds of macroporous resin and static adsorption curve of F resin ; density and pH of the extract liquor were investigated by use of factorial exeriment; HPLC fingerprint of extract of Herba Artemisiae scopariae were evaluated the adsorption effect. RESULTS ： The adsorpting effect were different among types of macroporous resin, and the adsorpting equilibrium time was 6 h in the use of F resin; Density and pH of the extract liquor are important factors of adsorption ; The ex-periment indicated that decreased liquor by this process were important factors of adsorption; The experiment indicated that this process decreased the yield of extract by 2.5%. CONCLUSION： The result can offer information about the determination of refinement process of extract of Herba Artemisiae scopariae with macroporous resin.
出处
《中成药》
CAS
CSCD
北大核心
2007年第2期199-202,共4页
Chinese Traditional Patent Medicine
基金
广东省中医药局科研课题
广东省科技计划项目(2004B10401007)
关键词
绵茵陈
大孔树脂
静态吸附
工艺
Herba Artemisiae scopariae
macroporous resin
static adsorption
process
