摘要
TLR4通过识别病原体而激活免疫细胞,在先天免疫和适应性免疫防御中起着重要作用。以中国荷斯坦奶牛、三河牛和中国西门塔尔牛共397头为研究对象,利用创造酶切位点PCR法扩增243 bp的目的片段,通过限制性内切酶HinfⅠ酶切来检测TLR4第3外显子的多态性,结果发现扩增产物的27 bp处C到T的突变使得多态位点产生,编码的氨基酸由苏氨酸变为异亮氨酸。A、B 2个等位基因在3个群体中均有分布,A等位基因占优势(大于78%),经χ2适合性检验,三河牛在该位点未达到Hardy-Weinberg平衡状态(P<0.05)。运用SAS 8.2软件采用最小二乘法拟合线性模型,将该基因座不同基因型与奶牛乳房炎进行了关联分析,结果表明:AA基因型为乳房炎抗性基因型(P<0.05),A等位基因为乳房炎抗性的有利基因。
Toll-like receptor 4 （TLR4） performed two functions： recognition of pathogen ligands and signaling to initiate innate and adaptive immune responses. In this experiment, a 243bp segment of the exon3 of TLR4 gene of Chinese Holstein, Sanhe cattle and Chinese Simmental was amplified by created restriction site PCR. The genetic polymorphisms of three populations were detected by digestion with restriction endomuclease Hinf Ⅰ. After sequencing, a polymorphic site in amplified production was identified of having either a C and a T at position 27 bp, which induced that Thr changed into Ile. Statistical results of χ^2 test indicated that the polymorphism locus in Sanhe cattle did not fit with Hardy-Weinberg equilibrium（P〈0.05）. Meanwhile the effect of polymorphism of TLR4 gene on somatic cell score was analyzed,the results showed that： the somatic cell score of individual with genotype AA were lowest significantly than that BB genotype（P〈0. 05）. In a word, the allele A might play an important role in mastitis resistance in bovine.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第2期120-124,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
"十五"国家重大科技专项(2002BA518A14)
国家"863"高技术研究发展计划项目(2002AA242011)
