摘要
以提高产绿链霉菌(Streptomyces viridochromogenes)SV-1产阿维拉霉素(Avilamycin)产量为目的,采用低能氮离子注入技术,辅之以链霉素抗性筛选法进行诱变选育研究。结果表明,“马鞍”区域即注入剂量范围在3×10^(15)～5×10^(15)ions/cm^2诱变效果最佳,菌株的抗药性突变与产量突变密切相关,链霉素抗性筛选法具有可行性。在摇瓶条件下,最终获得稳定性良好,阿维拉霉素产量达到83．5mg/L,较出发菌株提高195%的突变株SVT-45。实验表明,离子注入技术是一种有潜力的微生物诱变育种新方法。
To obtain high-yield avilamycin-producing strains, low energy N ^＋ ion implantation technology and screening of streptomycin-resistant mutants are used in the study on breeding mutation. The results show that, ＂saddle＂ region, which range is from 3 × 10^15 to 5 × 10^15 ions/cm^2, has got better induced mutation action. It also means that the strain＇s resistant mutation and yield mutation closely correlate to each other, and the method of streptomycin resistant screening is feasible. We have isolated a high-yield strain SVT-45 which the productivity is 195% higher than the original strain＇s in the rotation-flask experiments. These results showed that the ion implantation was an effective method for microbe mutagensis.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第1期39-42,共4页
Microbiology China
关键词
离子注入
诱变选育
链霉素抗性筛选
阿维拉霉素
Ion implantation, Mutation breeding, Screening of Streptomycin-resistant mutants, Avilamycin
