摘要
目的分析神经梅毒患者外周血中淋巴细胞、树突状细胞的亚群特点,探讨其与神经梅毒发病之间的关系。方法分别取健康人(10例,对照组)和神经梅毒患者(8例,试验组)外周血,用荧光标记法经流式细胞仪检测CD4+T、CD8+T、Th1、Th2细胞及DC1、DC2细胞的比例。结果神经梅毒患者外周血中的CD4+T细胞比例高于正常对照组(P<0.05);CD8+T细胞比例低于正常对照组(P<0.05)。在CD4+T细胞中,神经梅毒患者外周血中的Th1、Th2细胞分别占(14.12±5.12)%、(1.52±0.88)%,Th1/Th2比例为(12.05±5.62);正常对照组Th1、Th2细胞分别占(26.10±4.98)%、(0.99±0.35)%,Th1/Th2比例为(31.62±16.62)。神经梅毒患者外周血中的Th1细胞及Th1/Th2的比例低于正常对照组(P<0.05)。神经梅毒患者外周血中的DC1、DC2细胞分别占(0.44±0.17)%和(0.25±0.19)%,正常对照组分别占(0.34±0.10)%和(0.26±0.13)%,两组间差异无显著性意义(P>0.05)。神经梅毒患者的DC1与Th1、DC2与Th2存在相关关系(P<0.05),DC1、DC2与Th1/Th2无相关关系(P>0.05)。结论神经梅毒患者细胞免疫功能受抑,其外周血中DC亚群的比例与Th1/Th2失衡无关。
Objective To analysis the subsets of the lymphocyte and dendritic cells （DC） in peripheral blood in patients with neu- rosyphilis and investigate the relationship between these cells and onset of neurosyphilis. Methods The subsets of CD4^＋ T cells as well as DCs and the counts of CD4^＋ T and CD8^＋ T were analyzed by flow cytometry with immunofluorescent staining in 8 patients with neurosyphilis and 10 healthy controls. Results In peripheral blood of neurosyphilis patients the proportion of CD4^＋ T cell was significantly higher than that in the normal controls （ P 〈0.01） ; the proportion of CD8^＋ T cell was significantly lower than that in the normal controls （P 〈0.05）. The proportion of Th1, Th2 and the ratio of Th1/Th2 in neurosyphilis patients was （14. 12± 5.12） % , （1.52±0.88） % and （12.05±5.62）, respectively. In normal controls, it was （26.10± 4.98）% , （0.99±0.35） % and （31.62± 16.62） respectively. The expression of Th1 and the ratio of Th1/Th2 in neurosyphilis patients was significantly lower than the normal controls （ P 〈0.05）. The proportion of DC1 and DC2 in neurosyphilis patients was not significantly different from that in normal controls （ P ±0.05）. There was a significant correlation between DC1 and Th1, DC2 and Th2 in the neurosyphilis patients （ P 〈0. 051）, but no correlation between the proportion of DCs and the ratio of Th1/Th2 （ P 〉0.05）. Conclusion The cellular immune function of neurosyphilis patients may be inhibited, and there is no relationship between the proportion of DCs subset and unbalance of Th1/Th2. Key words：
出处
《中国康复理论与实践》
CSCD
2007年第2期183-185,F0003,共4页
Chinese Journal of Rehabilitation Theory and Practice
基金
首都医科大学基础-临床科研合作基金资助项目
