摘要
近年来,广西沿海在5—6月份常发生文蛤大批死亡现象,从死亡文蛤中分离到病原菌,经人工感染试验得到证实。病原菌为革兰氏阴性短杆菌(0.8-1.0×1.5-1.8μm),具偏端生单鞭毛。在TCBS琼脂平板上培养24h后,形成蓝绿笠状菌落,菌落直径2-3mm,发酵葡萄糖产酸不产气,精氨酸-碱反应阴性,赖氨酸、鸟氨酸脱羧阳性,靛基质阳性。在温度10-42℃、pH值5-11、盐度0.5-81%的环境条件下都能生长。该菌具有较强的毒力。经系统生理生化特性鉴定和VITEK微生物自动分析仪鉴定,病原菌为副溶血弧菌(Vibrioparahaemolyticu)。选择适当的养殖场地、做好蛤苗投放工作、掌握好放养密度、加强养成管理、加强环境监测预报是防治文蛤疾病流行的主要措施。
A high mortality of hard clam Meretrix meretrix occured alongthe beach of Guangxi Proyince during May and June in recent years.Pathogenic bacterium was isolated from. died clams, and it was confirmedto be the cause of the disease by infection experiment. The bacteriumshowed Gram negative shot rod with a deviate, single polar flagellum.It grew well and formed greenblue colony on TCBS agar. It could ferment glucose and produce acid but no gas. Tests for the deiarboxylationof lysine and ornithine were positive and arginine negatiye. Theisolate could grow well at temperature of 10-42℃, PH from 5 to 11 andNaCl of 0.8-8%. It is of high toxicity. The pathogen was identified asthe Yibrio parahaemolyticus. The main measures to control the diseasewere to select a suitable clam farm, strengthen the scientific management and keep the culture environment under disease inspection and forecast.
出处
《微生物学杂志》
CAS
CSCD
1996年第4期1-5,共5页
Journal of Microbiology
关键词
蛤
文蛤
副溶血弧菌病
Clam
meretrix meretrix
vibrio