摘要
目的观察病程为3个月的糖尿病大鼠神经及血管网膜超微结构的病理改变以及滋肾健脾化瘀片对其的影响。方法链脲佐菌素糖尿病大鼠随机分为模型组、导升明组、中药低剂量组、中药高剂量组,并设正常对照组,每日灌药1次,3个月后取后极部视网膜行消化酶铺片及透射电镜观察。结果糖尿病大鼠在3个月时视网膜血管出现管径粗细不均、排列紊乱、扭结和血管襻、未见微血管瘤,血-视网膜外屏障改变。神经网膜视细胞外节膜盘结构模糊,排列紊乱,肿胀变性,甚至溶解破坏。内节含有大量空泡样变的线粒体。内核层细胞内线粒体空泡样变增多。节细胞层核膜不连续、断裂,细胞核皱缩。中药组相对导升明组基底膜增厚减轻,血-视网膜外屏障紧密连接小带连接紧密,色素上皮的顶部微绒毛排列整齐;视细胞外节膜盘、内节线粒体、内核层及神经节细胞层都显示变性线粒体比例下降。结论病程3个月的糖尿病大鼠视网膜血管及神经网膜已出现病理改变,滋肾健脾化瘀片对糖尿病大鼠的视网膜血管及神经网膜具有保护作用,并能减轻血-视网膜外屏障的病理损害。
Objective To investigate the. efficacy of Zishenjianpihuayu pills in treatment of early diabetic retinopathy in rats after a 3 months duration of diabetes. Methods There were 50 healthy adult SD rats, 10 rats were nomud controls, 40 rats were injected intraperitoneally with 60 mg/kg Strep- tomtocin dosage, the Streptotacim -Diabefic rats were ＆vialed equally into four groups and treated differently with 2 ml suspension of low or high dose of zisbenjianpibuayu pills （0.6 g/kg,2.4g/kg）, 2 mi suspension of doxium （0.1 g/kg）, 2 ml distilled water once a day. After 3 months, the retinal Izypsin digest stretched preparation and uhrathin section were observed by light microscope or trasmission election microscope. Results The retinal vessel became dilating and torbaose, kinlding and looping but no microa- neurysm, there also had the breakdown of the outer blood-retina barriers in Streptozotocin-Diabetic rats. The acromere membranous disc were swelled, degenerated even dissolved, inner segment, inner nuclear layer, ganglion cell layer had much meuolus mitochondria. The lesions treating with Zishen- jienpilamyu pills （the basement membrane＇ s thiekening, breakdown of the outer blood-retina barriess,vacuolus mitockndria in inner segment,itmer nudeer layer, ganglion cell layer） were lessen than with daftium. Conclusion The retinal vessel and neurosensory retina of Streptozatocin-Diabetic rats have detected early lesions after a 3 months duration of diabetes. Treatment with Zishenjianpihuayu pills may be helpful in protecting the retinal vessel, neuretina and the outer blood-retina barriers.
出处
《眼视光学杂志》
2007年第1期45-47,共3页
Chinese Journal of Optometry & Ophthalmology
基金
广东省中医药管理局中医药
中西医结合科研计划基金项目(96039)
