摘要
目的:观察温补脾肾、泄浊化瘀药物对慢性肾功能衰竭(CRF)患者白细胞黏附分子(CD44,CD54)、肿瘤坏死因子(TNF-α)的影响,进一步探讨温补脾肾、泄浊化瘀法治疗慢性肾功能不全的作用机制。方法:将50例慢性肾功能衰竭患者随机分为治疗组28例、对照组22例,治疗组口服中药合剂,对照组口服尿毒清冲剂。采用流式细胞仪检测,用微量全血直接单标记荧光染色法、双抗体夹心ELISA法,观察治疗前后患者CD44,CD54,TNF-α及肌酐(SCr)、尿素氮(BUN)、血红蛋白(Hb)的水平变化。结果:治疗后2组CRF患者CD44,CD54,TNF-α水平及SCr,BUN均降低;Hb升高。治疗组与对照组比较差异显著(P<0.05)。结论:温补脾肾、泄浊化瘀药有降低CRF患者CD44,CD54,TNF-α水平的作用,并可改善SCr,BUN,Hb程度,减轻CRF患者的肾功能损害,对肾功能有保护作用。
Objective： To observe the effect of Wenbu Pishen, Xiezhuo Huayu therapy on cell adhesion molecule CD44, CD54, TNF-α in patients with chronic renal falure （CRF） and further study the mechanism of Wenbu Pishen, Xiezhuo Huayu therapy in treating CRF. Method： Fifty patients with CRF were divided into two groups randomly. The treatment group had 28 patients who were treated with oral Chinese herbal medicine mixture, and the control group had 22 patients who were treated with Niaeduqing. Using flow cytometry, microsample of whole blood direct single marker fluorescent staining analysis and double antibody sandwich ELISA were used to observe the before and after treatment changes of CD44, CD54, TNF-α, creatinine （SCr）, urean-N and hemoglobin （Hb）. Result： After the treatment, the contents of CD44, CD54, TNF-α, SCr and BUN were decreased in both groups, otherwise, Hb was increased. There was a significant difference between the treat group and the control group （P 〈 0. 05 ）. Conclusion： Wenbu Pishen, Xiezhuo Huayu therapy can decrease the level of CD44, CD54, TNF-α, improve the other relevant indexes, alleviate the damage of kidney, and have a protective effect on the kidney in patients.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第2期140-142,共3页
China Journal of Chinese Materia Medica
基金
黑龙江省中医管理局基金项目(200247)
