摘要
在实验室条件下研究了葡萄糖等10种碳源和组氨酸等11种氮源营养对大豆炭疽病菌菌丝生长速率和菌丝干重的影响。结果表明,葡萄糖、果糖、蔗糖、甘油、山梨醇、麦芽糖、半乳糖、淀粉和甘露醇等9种供试碳源均可被病原菌利用,但病原菌对不同碳源的利用程度有显著差异,其中葡萄糖作碳源时菌丝生长速率最快;果糖作碳源时菌丝干重最大。乳糖作碳源时菌丝生长速率最慢,菌丝干重最小,说明病原菌对乳糖的利用率低。组氨酸、苏氨酸、胱氨酸、丙氨酸、甘氨酸、硝酸钾、亚硝酸钠、精氨酸和蛋白胨等9种氮源均可被病原菌利用,综合考察平板菌丝生长速率与液体培养菌丝干重两个指标,以丙氨酸、组氨酸、苏氨酸、精氨酸和硝酸钾为病菌菌丝生长的较好氮源。硫酸铵和氯化铵对病原菌的菌丝生长有一定抑制作用。
The effects of different carbon and nitrogen sources of nutriment on the mycelial growth rates and the dry mycelial weight of Colletotrichum truncatum, the pathogenic fungus causing soybean anthracnose were studied respectively. The results showed that glucose, fructose, sucrose, glycerol, sorbitol, maltose, galactose, starch and mannite could be utilized by the pathogenic fungus. But there were difference in the effects of the pathogen to utilize different carbon sources. The mycelial growth of the fungus was the most quick with glucose as carbon source, and the dry weight of mycelium was the biggest with fructose as carbon source. But with lactose as carbon source, the mycelial growth of the fungus was the most slower and the dry weight of mycelium was the least, suggesting the pathogen couldn＇t utilize the carbon source effectively. Histidine, threonine, cystine, glycine, alanine, peptone, KNO3, and arginine could be all utilized by the pathogenic fungus. Among them, histidine, threonine, arginine and KNO3 were better for the mycelial growth of the pathogen, considering the integrated effects of both the mycelial growth rates and the dry mycelial weight. However, （NH4）2SO4 and NH4Cl seemed inhibitory against the mycelial growth of the pathogenic organism.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2007年第1期107-110,共4页
Journal of Anhui Agricultural University
基金
安徽省高校学科拔尖人才基金(200322)资助
关键词
大豆炭疽病菌
碳源
氮源
菌丝生长速率
菌丝干重
Colletotrichum truncatum
carbon sources
nitrogen sources
mycelial growth rates
dry mycelial weight
