摘要
为探讨米曲霉固体发酵生产中性蛋白酶的产酶条件,以米曲霉Y10为试验菌株,研究了产中性蛋白酶活力较高的固体培养基配方.结果表明:米曲霉产中性蛋白酶的适宜培养条件为:m(麸皮)∶m(酵母粉)∶m(乳糖)∶m(水)=9∶1∶1∶9,pH值为7.0,接种量为每克培养基接种1.8×108个孢子,最佳培养温度为30℃,最佳培养时间为72h.在此培养条件下,最高酶活力达4286.1U/g.
using Aspergillus oryzae Y10 as the test strain,medium for neutral protease solid fermentation was studied and optimized.The suitable composition （%） of the mediunl is as follows： wheat bran 9, yeast powder 1, lactose 1, water 9 , pH 7. 0. The optimal cultural temperature is 30℃, culture duration 72 hours, and inoculating 1.8 ×10^8 spores each gram medium. Under this optimal conditions, the neutral protease activity is as high as 4286.1 U/g.
出处
《玉林师范学院学报》
2006年第5期86-89,共4页
Journal of Yulin Normal University
关键词
米曲霉
固体发酵
中性蛋白酶
Aspergillus oryzae
solid fermentation
neutral protease
