摘要
取18日龄鸡胚，研究鸡肠上皮细胞（IEC）分离及原代培养的方法。结果表明：较好的分离条件是肠组织经0.1g／L中性蛋白酶和300U／mL胶原酶Ⅺ联合消化；较佳的培养条件是细胞在含2．5％～5％胎牛血清的DMEM培养基中，39℃、5％～7．5％CO2下培养，IEC可在1～2d贴壁，6～7d明显增殖，11～12d汇合成片。
18-day-old ehiek embryos were used to establish the method for isolation and primary eulture of the chick intestinal epithelial eell（IEC）. The results indicated that better isolation procedure for IEC from intestinal tissue was based upon a mixture of 0. 1 g/L neutral protease and 300 U/mL eollagease Ⅺ. The better culture eondition was in Dulbecco＇s Modified Eagle Medium eontaining 2.5%-5% foetal calf serum at 39℃ in a 5%-7.5% CO2 incubator. The IECs attached within 1-2 days,proliferated signifieantly within 6-7 days,and reached confluence as patches within 11-12 days under above condition.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2007年第1期74-76,80,共4页
Chinese Journal of Veterinary Science
基金
国家"985工程"重点建设项目(Y1101)
宁夏大学科学研究基金资助项目(05KF01)
关键词
肠上皮细胞
分离
原代培养
鸡胚
intestinal epithelial cell
isolation
primary culture
chick embryo
