摘要
对多胚水稻(Oryza sativa L.)ApⅢ的大量成熟颖果、人工萌发的幼苗和开花后3~5 d 的幼嫩颖果进行的整体解剖和显微制片观察表明:ApⅢ的5000粒成熟颖果中,89.0% 含单胚单苗,8.9% 和1.2%分别含双胚双苗和三胚三苗;700多粒幼嫩颖果中,90.0% ~95.0% 含单胚,5.0% ~7.0% 含双胚。因制片的数目有限,未见到含三胚的;在含单胚和多胚颖果中,胚均位于同一胚囊的珠孔端,未见到胚囊以外存在不定胚。根据上述结果。
Since 1988s, the phenomenon of multiple seedlings and multiple embryoes of apomictic rice,ApⅢ(Shuang 13) has been interestingly concerned.Embryological investigation of ApⅢ has been carried out with the whole dissection and conventional sectioning technique.The main conclusions are as follows:1.8.9% of 5000 matured caryopsises, contained one embryo in a caryopsis,from which single seedling arose,while 8.9% twin embryoes and 1.2% triplet embryoes of which developed two seedlings and triple seedlings respectively;2.In over 700 young caryopsises,90.0%~95.0% contained only one embryo,5.0%~7.0% twin embryoes,except a few of the ovules with absorptive embryo sac or embryo and endosperm.Triplet embryoes caryopsis could not be traced further due to their low frequency;3. Authors' materials showed that all the single,twin and triplet embryoes were situated at the micropylar end in one embryo sac of young ovule and no adventitious embryo was seen outside the embryo sac of ovules.From the aforementioned results,it can be infered that albeit single embryo is derived from the egg cell yet it could be possible twin or triplet embryoes may be developed from the egg cell and one or two of the synergids either or not through fertilization.
基金
中国科学院院长基金