摘要
研究空心莲子草(Alternanthera philoxeroides)杀灭钉螺的作用机理。将空心莲子草水浸液(1g/100ml)及去氯水分别浸泡钉螺24h后,用透射电镜观察两组钉螺神经节及足肌超微结构的变化。结果显示:钉螺经空心莲子草水浸液浸泡24h后,神经节细胞明显破坏,其细胞膜消失,细胞核固缩,胞质内细胞器变性坏死,次级溶酶体增多,神经纤维断裂;足肌纤维内细胞核肿胀,肌丝排列紊乱甚至溶解,细胞间隙明显增宽。结果提示:空心莲子草水浸液对钉螺神经节细胞有明显破坏作用,同时可引起足肌细胞变性及细胞间隙水肿。
To study the mechanisms of molluscicide from Alternanthera phliozeroides snails, Oncomelania hupensis, were immersed in aqueous extract of Altemanthera philozeroides or in dechlorinated water for 2.4h. Ultrastructure of the ganglion and foot muscle from the immersed snails were observed by transmission electron microscopy（TEM）. Results of experiments demonstrated that after 24 h exposure to aqueous extract of Altemanthera philoxeroides, ganglioneures was evidently damaged, its pericellular membrane disappeared, nucleus were pycnosis, cell organs denatured and necrosized, secondary lyscome increased and nerve fiber broken, and in the meanwhile, nucleus of foot-muscle cells swelled and gap between cells broadened. These ultrastructural changes indicated that the aqueous extract of Alternanthera philoxeroides had an obviously destruction of ganglioneures and caused foot-muscle cells to denature and a hydrops in between cells.
出处
《电子显微学报》
CAS
CSCD
2006年第6期524-527,共4页
Journal of Chinese Electron Microscopy Society
基金
国家自然科学基金资助项目(No.30570322
No.30170179)
湖北大学中药生物技术省重点实验室开放基金(2004-2006).~~
关键词
空心莲子草
钉螺
神经节
足肌
透射电镜
Alternanthera philoxeroides
Oncomelania hupensis
ganglioneure
foot muscle
transmission electron microscopy
