摘要
目的：考察高效液相色谱中流动相pH值的变化对氢化可的松琥珀酸钠保留行为的影响。方法：色谱柱为Varian C18，流速为1．0ml·min^-1，柱温为40℃，检测波长为242nm，进样量为20μL，流动相为甲醇-磷酸盐缓冲液（8mmol．L^-1磷酸二氢钾液，43：57，临用前用8mmol·L^-1磷酸氢二钾溶液调节pH值分别为5．0、5．2、5．6、6．0）。结果：流动相pH值的变化仅改变氢化可的松琥珀酸钠和游离氢化可的松的保留时间，不改变其出峰顺序，当pH值为5．2时氢化可的松琥珀酸钠峰形及与游离氢化可的松分离较好，对称度在0．95-1．05之间。结论：本方法可为常用药物色谱行为规律的基础性系统研究提供依据。
OBJECTIVE： To study the effect of pH value of mobile phase in HPLC on the retention behavior of hedrocor tisone succinate sodium.METHODS： The separation was performed on Varian C18 with the column temperature at 40℃. The mobile phase consisted of methanol - phosphate buffered solution（43 ： 57） with（8mmol · L^-1 KH2PO4 solution, the pH value of the mobile phase was adjusted to 5.0, 5.2, 5.6 and 6.0, respectively with 8mmol · L^-1 K2HPO4 solution） flow rate at 1.0mL · min^- 1,detection wavelength at 242nm and sample size at 20μL. RESULTS： The pH value of mobile phase could only change the retention time of hydrocortisone sodium succinate and hydrocortisone, but not their peak occurring order.At a pH value of 5.2, both hydrocortisone sodium succinate and free hydrocortisone separated well and their peaks were in good shape, with symmetry value at 0.95-1.05.CONCLUSION： This method can provide bases for the basic systematic study of chromatographic behaviors of commonly used drugs.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第1期57-58,共2页
China Pharmacy
