摘要
目的:观察苦豆子总碱对实验性结肠炎大鼠的外周血和结肠组织中调节性T细胞CD4+CD25+,CD8+CD28-表达的影响。方法:实验于2006-03/08在南方医科大学实验室进行。①分组:将72只SD大鼠数字表法随机分成正常对照组,模型组,5-氨基水杨酸组,苦豆子15,30,60mg/kg组共6组,每组12只。②造模:除正常对照组外,其他5组采用三硝基苯磺酸灌肠法制备结肠炎大鼠模型,正常对照组给予等量生理盐水灌肠。③给药:造模第2天开始灌胃给药,2mL/只,1次/d。5-氨基水杨酸组灌胃5-氨基水杨酸400mg/kg,苦豆子15,30,60mg/kg组灌胃酸苦豆子总碱15,30,60mg/kg,其他2组灌等量生理盐水。④取材:在第1周和第3周各组分别取6只大鼠处死,测定结肠黏膜组织和外周血中CD4+CD25+,CD8+CD28-的表达水平,观察苦豆子总碱对模型大鼠急慢性期T细胞亚群的影响。结果:72只大鼠进入结果分析。①建模第1周:模型组结肠内T细胞亚群CD4+CD25+,CD4+CD25+/CD25-,CD8+CD28-及CD8+CD28-/CD28+高于正常对照组(P<0.05,0.01),苦豆子各剂量组数据虽低于模型组,但经统计学处理后差异不显著(P>0.05)。②建模第3周:模型组大鼠外周血中的CD8+CD28-和CD8+CD28-/CD28+的表达高于正常对照组和苦豆子30,60mg/kg组[CD8+CD28-:(11.3±1.3)%,(5.4±2.2)%,(5.8±3.2)%,(6.0±4.2)%;CD8+CD28-/CD28+:(1.2±0.8)%,(0.4±0.1)%,(0.5±0.2)%,(0.5±0.4)%;P<0.05,0.01];结肠组织中的CD8+CD28-和CD8+CD28-/CD28+的表达也高于正常对照组和苦豆子30,60mg/kg组[CD8+CD28-:(17.4±4.2)%,(3.8±4.5)%,(3.9±4.0)%,(4.2±3.6)%;CD8+CD28-/CD28+:(1.8±0.3)%,(0.7±0.3)%,(0.7±0.2)%,(0.7±0.3)%;P<0.05,0.01]。结论:苦豆子总碱30,60mg/kg能下调实验性结肠炎大鼠慢性期高表达的CD8+CD28-T细胞。
AIM： To investigate the effects of total base of Sophora alopecuroides on the expressions of CD4^±＋CD25^＋, CD8^＋CD28^- Tregs in the peripheral blood and colon tissues of rats with experimental colitis. METHODS： The experiment was conducted in the Laboratory of Southern Medical University from March to August 2006. ① Grouping： 72 SD rats were randomized into 6 groups： Normal control group, the model group, 5- aminosalicylic acid （5-ASA） group and 60 mg/kg, 30mg/kg, 15mg/kg Sophora alopecuroides group With 12 rats in each group.② Modeling： Rats in all groups except those in the normal control group were induced into models of colitis by enema of trinitro-benzene-sulfonic acid （TNBS）, while rats in the normal control group were given enema of normal saline at the same dose. ③Administration： Rats began to receive intragastric administration from the second day once a day and 2 mL for each rat. Rats in the 5-ASA group were given gastric perfusion of 5-ASA at 400 mg/kg, and rats in 15 mg/kg, 30 mg/kg and 60 mg/kg Sophora alopecuroides groups were given gastric perfusion of the total base of Sophora alopecuroides respectively for 15, 30 and 60 mg/kg, while rats in the other two groups were gastrically perfused with normal saline at the same dose. ④ Material-drawing： Six rats were executed in each group respectively at the first and third weeks to measure the expressions of CD4^＋CD25^＋ ,CD8^＋ CD28^- in the colon mucous membrane and peripheral blood, so as to study the effect of total base of Sophora alopecuroides on T cell subgroup in colitis rats at acute or chronic phase. RESULTS： A total of 72 rats were involved in the analysis of results. ① In the first week of modeling： The proportion of CD4^＋CD25^＋,CD4^＋CD25^＋/ CD25^-,CD8^＋CD28^- and CD8^＋CD28^-/CD28＋ Tregs on the colon of colitis rats in the model group was higher than that in the normal control group （P 〈 0.05, 0.01）, and it was lower in the Sophora alopecuroides group at all doses than that in the model group, while the statistical differences were not significant after the treatment （P 〉 0.05）. ② In the third week of modeling： The expressions of CD8^＋CD28^- and CD8^＋CD28^-/CD28^＋ Tregs in the peripheral blood of rats in the model group were obviously higher than that in the normal control group and 30 mg/kg, 60 mg/kg Sophora alopecuroides groups [CD8^＋CD28^-： （11.3±1.3）%, （5.4＋2.2）%, （5.8±3.2）%, （6.0±4.2）% ; CD8^＋CD28^-/CD28^＋ ： （ 1.2 ±0.8 ）%, （0.4 ±0.1 ）%, （0.5 ±0.2）%, （0：5±0.4）% ;P 〈 0.05,0.01], and the expressions of CD8＋CD28 and CD8^＋CD28^-/CD28^＋ in the colon tissues were higher than those in the normal control group and 30 mg/kg and 60 mg/kg Sophora alopeeuroides groups [CD8^＋CD28^-： （17.4±4.2）%, （3.8±4.5）%, （3.9±4.0）%, （4.2±3.6）% ; CD8^＋CD28^-/CD28^＋ ： （ 1.8±0.3 ）%, （0.7±0.3）%, （0.7±0.2）%, （0.7±0.3）%;P 〈 0.05,0.01]. CONCLUSION： Total base of Sophora alopeeuroides can down-regulate high expressions of CD8^-CD28^- Tregs of colitis rats at chronic phase.
出处
《中国临床康复》
CSCD
北大核心
2006年第47期89-91,94,共4页
Chinese Journal of Clinical Rehabilitation
基金
广州市科技计划项目资助(2006Z3-E5051-2)~~
