摘要
测定了兰州鲇(Silurus lanzhouensis)胃、肝胰脏、前肠、中肠、后肠在不同温度(15℃、20℃、25℃、30℃、37℃4、2℃、47℃)条件下的蛋白酶、淀粉酶和脂肪酶的活性。结果表明,随温度的升高,各种酶活性的变化均表现为先增高后下降直至不能检出。消化道各部位蛋白酶的最适温度均为42℃;淀粉酶的最适温度除胃和肝胰脏为37℃外,其他部位均为30℃;脂肪酶的最适温度除后肠为30℃外,其他部位均为25℃。消化酶的最适温度高于其生活水域的水温,反映出消化酶作为酶蛋白的耐热性。最适温度下,蛋白酶活性前肠≈中肠>后肠>肝胰脏≈胃;淀粉酶活性前肠>中肠>肝胰脏>胃>后肠;脂肪酶活性中肠>后肠>前肠≈肝胰脏>胃。研究结果还表明,前肠和中肠是兰州鲇消化蛋白的主要部位,中肠是其消化脂肪的主要部位,而前肠是其消化淀粉的主要部位。在消化酶表现出活性的温度范围内,蛋白酶活性明显高于淀粉酶活性。实验还表明脂肪酶具有活性的温度范围较蛋白酶和淀粉酶窄。
The protease, amylase and lipase activities in Silurus lanzhouensis under the condition of different temperature （15℃ , 20℃, 25℃ , 30℃, 37℃ , 42℃ and 47℃ ） were studied. The results showed that： with the increasing of temperature, the digestive enzymes＇ activities increased at first, then decreased. The optimum temperatures of the protease in all digestive tissues and organs were 42℃ ; those of amylase were 30℃ except stomach and hepatopancreas,which was 37℃ ; those of lipase were 25℃ except posterior intestine, which was 30℃ . The optimum temperatures of these enzymes were higher than the temperature that the fish lived in, which showed the protein of enzymes＇ thermostability. Under respective optimum temperature, protease activity changed in the order from high to low as anterior intestine ≈ middle intestine 〉 posterior intestine 〉 hepatopancreas ≈ stomach ; amylase activity changed as anterior intestine 〉 middle intestine 〉 hepatopancreas 〉 stomach 〉 posterior intestine ; lipase activity changed as middle intestine 〉 posterior intestine 〉 anterior intestine ≈ hepatopancreas 〉 stomach, which showed that anterior intestine and middle intestine are the main positions in digesting protein, also anterior intestine and middle intestine are the main positions in digesting amylase and lipid respectively. The activity of protease is higher than that of amylase in the temperature from 25 ℃ to 37 ℃ . The result also showed that the range of temperature for lipase to exert its activity was narrower than that of protease and amylase.
出处
《动物学杂志》
CAS
CSCD
北大核心
2006年第6期104-108,共5页
Chinese Journal of Zoology
基金
中国水产科学研究院科研基金项目(No.2003-01-05)
关键词
温度
兰州鲇
蛋白酶
淀粉酶
脂肪酶
活性
Temperature
Silurus lanzhouensis
Protease
Amylase
Lipase
Activity
