摘要
目的采用大鼠局灶性脑缺血再灌注模型,观察右-苯丙胺对脑缺血损伤的保护作用。方法应用Koizum i线栓法建立单侧局灶性脑缺血再灌注模型(m idd le cerebral artery occlusion,MCAO)模型。术后1、3、6周应用原位末端标记法观察细胞凋亡并计数阳性细胞,免疫组织化学方法及RT-PCR检测缺血周围区生长相关蛋白(GAP-43)表达的变化。结果右-苯丙胺治疗组较自然恢复组细胞凋亡明显减少;免疫组化光密度定量测定及RT-PCR显示GAP-43的表达水平在第1周末明显增高,且治疗组高于自然恢复组。结论右-苯丙胺能减少脑梗死边缘区细胞凋亡的发生,促进GAP-43的表达。
Objective To observe the protection of D-amphetamine on rat brain after focal cerebral ischemia. Methods The unilateral middle cerebral artery occlusion （MCAO） models were established by using Koizumi＇ s method. TUNEL was applied to detect quantitatively brain cell apoptosis at 1st, 3rd and 6th week after operation. Immunohistochemical staining and RT-PCR were respectively used to detect the expression of growth-associated protein 43 （ GAP-43 ） and GAP-43 mRNA around ischemic area. Results Apoptosis of brain cells reduced evidently in the group treated with D-amphetamine. GAP-43 protein detection demonstrated statistically significant increase in immunoreaction product as determined by optical density measurements in D-amphetamine treated group compared with the group without any agent treatment. The same results appeared in RT-PCR product. Conclusion D-amphetamine can reduce brain cell apoptosis and promote GAP-43 expression.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第1期81-83,共3页
Journal of Third Military Medical University
关键词
脑缺血
生长相关蛋白43
右-苯丙胺
凋亡
brain ischemia
growth-associated protein 43
D-amphetamine
apoptosis
