摘要
目的探讨巴曲酶(Batroxobin,BX)对移植静脉内膜增生的影响。方法25只杂种犬分为实验组、对照组、假手术组分别行股静脉移植到股动脉,建立静脉移植模型。实验组给以巴曲酶治疗。分别检测3组血浆一氧化氮(nitric oxide,NO)、内皮素(endothelin,ET)含量,计算机图像分析测定移植静脉内膜、中膜面积及内膜面积／中膜面积比值,免疫组织化学染色检测移植静脉增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)和原癌基因C-myc表达情况。结果血浆NO含量实验组高于对照组和假手术组(P<0．01),ET含量实验组低于对照组和假手术组(P<0．01)。内膜面积和中膜面积在对照组较实验组和假手术组增生明显(P<0．05)。实验组与对照组比较,内膜面积/中膜面积之比值,差异无统计学意义(P>0．05)。PCNA表达实验组与对照组比较,差异有统计学意义(P<0．05),C-myc表达实验组与对照组比较,有差异有统计学意义(P<0．01)。结论巴曲酶对内膜增生机制中的部分因素起抑制作用,对血管内膜增生具有一定的防治作用。
Objective To study the effects of Batroxobin （BX） on the intimal proliferation of graft veins. Methods In this study 25 dogs were selected and evenly divided into experimental group, control group and sham operation group. In experiment and control group, a segment of auto- femoral vein were grafted into femoral artery by clean microsurgery technique. In experimental group, BX was given at the dosage of 0. 1 BU/kg, dayly × 2 preoperatively, and once a day for consecutive 6 days postoperatively. Plasma NO, ET was determined in the three groups. Computer image analysis system was applied to calculate the thickness of neointima and media in the vein grafts, immunohistochemistry was used to identify PCNA and C-myc. ＂Result The experimental group had a higher level of NO and lower level of ET compared with control group and sham operation group（ P 〈 0. 01 ）. The area of neointima and media of graft veins in the control group was higher than that in the experimental group and sham operation group （P 〈 0. 05）. The ratio of neointima area over media area was not statistically different in experimental group compared with that in control group （P 〉 0.05 ）. The PCNA expression in experimental group was statistically different from that of the control group（ P 〈 0. 05 ）. C-myc expression was higher in control group than that of experimental group （ P 〈 0. 01 ）. Conclusion BX can inhibit intimal hyperplasia to some degree. It could be helpful for the prevention and treatment of intimal hyperplasia.
出处
《中华普通外科杂志》
CSCD
北大核心
2006年第11期795-797,共3页
Chinese Journal of General Surgery
关键词
移植
自体
静脉
巴曲酶
血管内膜增生
基因
Transplantation, autologous
Veins
Batroxobin
Intimal proliferation
Genes
