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始旋链霉菌HCCB2003-8 snaA、snaB和snaC基因的克隆、序列分析及原核表达

Cloning,sequencing and prokaryotic expression of snaA,snaB and snaC gene from Streptomyces pristinaespiralis HCCB2003-8
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摘要 以始旋链霉菌(S trep tomy ces p ristinaesp ira lis)HCCB 2003-8的染色体DNA为模板,通过优化PCR条件扩增出FM N还原酶、P IIA合酶的基因片段。与文献报道的基因序列做同源比较,snaA和snaB碱基序列分别只有一个碱基的差异,snaC碱基序列完全匹配。将snaA、snaB和snaC的基因片段插入原核表达载体pET 30a中,并转化至大肠埃希菌BL 21(DE 3)。SDS-PAGE结果表明,经IPTG诱导后基因分别得到表达。 Through PCR method and optimization of PCR reaction system, snaA, snaB and snaC gene were obtained from Streptornyces pristinaespiralis HCCB2003-8. Analysis of snaA, snaB and snaC gene sequences showed that there was only one-base-difference from the reported sequences of snaA and snaB respectively, and snaC sequence was identical to the reported sequence, snaA, snaB and snaC gene were inserted into pET30a vector and were transformed into E. coli BL21 （DE3） strain. After induced by IPTG, the result of SDS-PAGE showed that they were expressed.
作者 王宁波 殷瑜 黄为一 朱丽 陈代杰
机构地区 南京农业大学微生物学系 上海来益生物药物研究开发中心
出处 《中国抗生素杂志》 CAS CSCD 北大核心 2006年第11期654-656,674,共4页 Chinese Journal of Antibiotics
关键词 始旋链霉菌 普那霉素 基因克隆 表达 Streptomyces pristinaespiralis Pristinamycin Gene cloning Expression
分类号 Q78 [生物学—分子生物学]
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参考文献5

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二级参考文献8

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中国抗生素杂志
2006年 第11期

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