摘要
建立高效液相色谱法测定了野山楂中乌索酸和齐墩果酸的含量,同时采用光电二极管阵列检测器检测两组分的紫外光谱。色谱柱为NucleosilC18柱(4.6mm×250mm,5μm),流动相为甲醇-水(87:13,V/V),流速0.8ml/min,检测波长210nm,柱温26℃。乌索酸在0.1244～2.488μg范围内线性关系良好(r=0.9996),平均回收率为98.1%。齐墩果酸在0.1192～2.384μg范围内线性关系良好(r=0.9999),平均回收率为101.2%。方法简便、准确、重现性好、线性范围宽,适用于测定野山楂中乌索酸和齐墩果酸的含量。
Adetenninafion method of ursolic acid and oleanolic acid in Crataegus cuneata Sieb. et Zucc. by reversed-phase high performance liquid chromatography was established and at the time UV spectrum of two components was detected by means of photodiode array detector（PAD）. The chromatographic cohnnn, Nucleosil C184.6m × 300m, 5μ m）, methanol- water （87： 13,V/V） mobile phase with 0.8ml/min flow rate, the detected wavelength （210nm）, and the column temperature（26℃） were adopted. The calibration curve of ursolic acid was linear under the content of 0.1244-2.488μg, the correlation coefficient was over 0.9996.The average recovery of ursolic acid was 98.1%. The calibration curve of oleanolic acid was linear under the content of 0.1192-2.384μg, the correlation coefficient was over 0.9999. The average recovery of oleanolic acid was 101.2%. The method is simple, accurate, reproducible and has wider linear range. The method can be used to determine ursolic acid and oleanolic acid in Crataegus cuneata Sieb. et Zucc.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第11期438-440,共3页
Food Science
基金
国家"863"计划重点资助项目(2002AA2Z3217)
关键词
野山楂
乌索酸
齐墩果酸
光电二极管阵列检测器
高效液相色谱
含量测定
Crataegus cuneata Sieb.et Zucc
ursolic acid
oleanolic acid
photodiode array detector
HPLC
content determination
