摘要
设计了原位电解池用于生物材料/活细胞界面电化学交流阻抗谱(EIS)的测量,原位观测了钛/MG63细胞界面EIS行为及其随时间的变化.原位电解池中引入Ag/AgCl作为参比电极,解决了EIS测量过程中高频部分信号失真的问题.钛/MG63细胞界面EIS研究初步结果表明:活细胞在钛电极表面附着,可改变界面双电层结构和电极的表面状态,进而影响界面的电化学性质.实验发现,由于钛电极表面氧化层的阻抗信号处于中低频段,而细胞膜层自身的电化学阻抗响应位于高频范围,由此,可分别分析不同的电极过程,解析其相互关系,研究活细胞在生物材料表面的作用机理.
Using a home-made in situ electrochemical impedance behaviors of the titanium/MG63 cells interface monitor the interactions of biomaterials and living cells in cell of electrochemical impedance spectroscop（EIS）, the were measured to elucidate the interfacial structure and to a physiological condition. The in situ electrochemical cell was designed to detect the dynamic EIS behavior of living cells growing on a tiatanium surface. A three-well design of electrochemical cell was used to decrease system error and a home-made Ag/AgCl electrode was introduced as a reference electrode to eliminate the distortion phenomena in high frequency range. The EIS spectra and its time dependency of titanium/MG63 cells interface showed that the polarization resistance was increased with the decreasing of CPE constant （Y0） and a new time constant in high frequency range was detected. It suggested that the adhesion and growth activities of the living cells on the biomaterial surface may influence the surface status and the structure of the double layer at the interface, and thus alter its electrochemical characteristics.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
2006年第10期1248-1252,共5页
Acta Physico-Chimica Sinica
基金
国家自然科学基金(20273055)
教育部博士点基金(20020384012)
国家863计划(2003AA302230)资助项目
