摘要
从农药厂废水处理池的活性污泥中分离到1株能以甲氰菊酯为唯一碳源生长的细菌,命名为JQL4-5.根据其生理生化特征和16S rDNA(GenBank Accession No.DQ177525)序列相似性分析,将该菌株鉴定为鞘氨醇单胞菌属(Sphingomonassp.).该菌株在24h内对20 mg/L的甲氰菊酯的降解率达到99.8%.降解甲氰菊酯的最适温度为30℃,pH为7.0,降解速率与初始接种量呈正相关.酶的定域试验表明,降解甲氰菊酯的酶为胞内酶.
A bacterium capable of utilizing fenpropathrin as sole carbon .source was isolated from activated sludge collected from wastewater-treating system of a pesticide manufacturer. This bacterium was identified as Sphingomonas sp. according to its physiological ＆ biochemical analysis and the similarity analysis of its 16S rDNA sequence （GenBank Accession No. DQ177525）. This bacterium could degrade 99.8% of 20mg/L fenpropathrin in 24h. The optimal pH and temperature for the degradation were 7.0 and 30℃, respectively. The degradation speed was related positively to initial inoculum size. The enzyme distribution experiment showed that the degrading-enzyme in the bacterium was endoenzyme.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2006年第10期2100-2104,共5页
Environmental Science
基金
国家自然科学基金项目(30400013
40471073)
江苏省科技厅项目(BG2005322)
土壤与农业可持续发展国家重点实验室开放基金项目(055112)
