摘要
骨保护素/NF-κB受体活化剂配体/NF-κB受体活化剂(OPG/RANKL/RANK)系统对破骨细胞生成、功能起着关键的作用,它的发现是骨生理代谢研究领域的重大进展。为研究生理性机械应力对鼠骨髓基质细胞OPG/RANKLmRNA表达变化的影响,进一步探讨机械应力对破骨细胞的影响机制,通过对鼠骨髓基质细胞施加不同时段的生理性的机械应力,并以RT-PCR半定量的方法检测OPG/RANKLmRNA表达变化趋势。结果显示随着加力时间的延长(6h后)RANKLmRNA表达减少34.4%,而OPGmRNA(9h后)表达增加73%,提示生理性应力能显著影响鼠骨髓基质细胞OPG/RANKLmRNA表达变化,从而在一定程度上阐明了生理性应力延缓骨质吸收的内在机制。
The recent identification of the receptor activator of NF-κ3 ligand （RANKL）, its cognate receptor RANK, and its decoy receptor osteoprotegerin （OPG） has led to a new molecular perspective on osteoclasts biology and bone homeostasis. To investigate the effect of physiological mechanical strain upon OPG/RANKL mRNA expression by murine stromal cells and further elucidate the mechanism of mechanical effect on osteoclasts, the OPG/RANKL genes expression was analyzed by semi-quantitative reverse-transcript polymerase chain reaction （RT-PCR） after time-controlled mechanical strain applied to murine stromal cells. The results showed that mechanical strain enhanced the expression of OPG genes to 73% after 9 h applying, while reduced the expression of RANKL genes to 34.4% after 6 h applying respectively. It is suggested that strain-induced OPG/RANKL genes expression tendency by murine stromal cells may contribute to explain the mechanism of osteolysis prevention by physiological mechanical strain.
出处
《细胞生物学杂志》
CSCD
2006年第5期747-751,共5页
Chinese Journal of Cell Biology
基金
国家自然科学基金资助项目(No.30274127)~~
