摘要
目的观察两种共刺激分子CD137及CD28对D-半乳糖致亚急性衰老模型小鼠及自然衰老小鼠T细胞活化后的细胞增殖、白介素2(IL-2)分泌及细胞存活的影响。方法7周龄BALB／c雄性小鼠随机分为D-半乳糖致亚急性衰老模型组(衰老模型组)、对照组和青年组,衰老模型组小鼠每日背部皮下注射D-半乳糖溶液(120 mg／kg,溶于0．1 ml蒸馏水中),连续注射5个月,建立亚急性衰老小鼠模型;对照组小鼠每日背部皮下注射0．1 ml蒸馏水,连续注射5个月;青年组小鼠未行处理。自然衰老组为16月龄BALB／c雄性小鼠。分离各组小鼠的脾脏T细胞,分别用ConA+ IgG、ConA+CD137单抗、ConA+CD28单抗体外活化,测定T细胞的增殖水平、培养上清液中IL-2的浓度及细胞凋亡率。结果(1)衰老模型组T细胞经ConA+IgG活化后,细胞增殖、IL-2的分泌水平(A值)及细胞存活率分别为0．422±0．057、0．632±0．066及68．0％±2．4％,与自然衰老组T细胞经ConA+IgG活化后的相应指标的差异无统计学意义,但两组衰老T细胞的活化指标均显著低于ConA+IgG活化的青年组及对照组;(2)衰老模型组T细胞应用ConA+CD137单抗活化后,细胞增殖、IL-2的分泌水平分别为0．639±0．053、1．119±0．035,显著高于ConA+IgG活化组,细胞存活率为53．3％±2．4％,显著低于ConA+IgG活化组,自然衰老组也显示同样的结果;并且发现CD137单抗对衰老T细胞功能的调节作用弱于CD28单抗。结论(1)衰老模型组与自然衰老组的T细胞功能均降低,呈现相似的增龄性变化;(2)CD137及CD28分子对两组衰老T细胞有近似的调节作用,均能促进衰老T细胞的活化及存活,CD28分子的调节作用强于CD137分子。
Objective To investigate the effect of co-stimulatory molecular CD137 and CD28 on the cell proliferation, IL 2 secretion and cell apoptotic rate of activated T cells in naturally senile mice and subacute senile mice induced by D-galactose. Methods Seven-week-old BALB/c male mice were divided into D-galactose induced subacute senile group （D-gal group）, control group and young group randomly. Subacute senile mice model was established by back hypodermic injection of D-galactose （120 mg/kg, dissolved in 0. 1 ml distilled water） everyday for five month. Control group was established by injection of 0.1 ml distilled water everyday for five month. Young group was injected with nothing. And 16-month-old BALB/c male mice was taken as aged group. The spleen T cells of each group were isolated and activated in vitro stimulation with ConA＋ IgG, ConA＋ CD137mAb or ConA＋ CD28mAb. The cell proliferation, apoptotic rate and IL-2 concentration in cell culture supernate of T cells were detected. Results （1） The cell proliferation（0. 422 ±0. 057, A）, IL-2 secretion（0. 632±0. 066, A）and apoptotic rate（68.0% ±2.4%） of T cells in D-gal group stimulated in vitro with ConA＋ IgG showed no significant difference when compared with those of aged group. Compared with young and control group, activation of T cell in D-gal and aged groups were significantly decreased; （2） Cell proliferation, IL-2 secretion and cell survival of T cells in D-gal group and aged group were significantly promoted by both ConA ＋ CD137mAb [-（ 0. 639 ± 0.053, A）, （1. 119±0. 035 ,A）, （53.3 ± 2. 4） %, respectively] and ConA ＋ CD28mAb. CD137 mAb had less effect on both groups than did CD28 mAb. Conclusions （1） Similar age-associated alterations happen in T cells of both D-gal group and aged group. （2） CD137 and CD28 can promote the activation and survival of T cells in aged and D-gal group. But CD28 has stronger effect on regulation of T cells than CD137.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2006年第9期687-690,共4页
Chinese Journal of Geriatrics
