摘要
为了研究己烯雌酚（DES）对胎鼠睾丸发育的影响，将雌鼠随机分为对照组和试验1、2、3组，每组10只。3个试验组母鼠从怀孕第10～18天分别每天连续皮下注射DES2、4和8μg／R，对照组则注射同等剂量的生理盐水，于最后一次注射后2h取出胎鼠睾丸。常规石蜡切片，HE染色镜检，并应用免疫组织化学方法检测雄性胎鼠睾丸中增殖细胞核抗原（PCNA）的表达情况。结果显示：2雌组睾丸发育情况接近对照组，无明显病理变化；4μg组仅见生殖母细胞和间质细胞数量减少；8μg组可见生殖母细胞核仁不明显，支持细胞数量减少，间质细胞溶解；随着用药剂量的增加，睾丸中PCNA含量显著降低（P〈0．05）。结果表明DES会抑制胚胎期睾丸细胞的增殖，从而影响睾丸的发育，破坏睾丸的组织结构。
The effects of diethylstilbestrol （DES） on testicle development of fetal mouse were studied in the experiment. Forty female mice were divided into 4 groups randomly, control group and 3 experimental groups. DES was injected hypodermically from 10 to 18 days at dosage of 2, 4 and 8 μg for each mouse per day after pregnancy while the control group was administrated with saline hypodermically. After 2 hours of the last drug administration, the testicle of embryo was collected and observed in the HE stain. Expression of proliferating cell nuclear antigen （PCNA） in testicle tissue was detected with immunohistochemistry. The results were showed as follows ： 1 ） Development of testicle was close to the control group in 2 g group. 2 ） There was decrease in amount of gonocyte and interstitial cell in 4 g group. 3 ） Nucleolus of gonocyte was not clear, amount of interstitial cell was reduced, sertoli cell was dissolved in 8 g group. 4） Content of PCNA was decreased as dosage of DES increased （ P 〈 0. 05 ）. All above indicated that the DES could inhibit the proliferation of orchic cells in embryo, then influenced the testicle development, damaged the testicle structure.
出处
《畜牧与兽医》
北大核心
2006年第7期1-4,共4页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(10572029)
关键词
己烯雌酚
胎鼠
睾丸发育
diethylstilbestrol
fetal mouse
development of testicle
