摘要
目的观察银杏提取物(EGb)对大鼠肾缺血再灌注后肾小管细胞凋亡调控基因bcl-2和bax表达的影响,探讨EGb肾保护作用的分子生物学机制。方法用动脉夹夹闭大鼠双侧肾蒂45 min再灌注24 h方法制成急性肾缺血再灌注损伤模型。采用脱氧核苷酸末端转移酶介导的DNA原位末端标记技术检测细胞凋亡的情况。免疫组化检测Bcl-2、Bax表达。结果缺血再灌注组较假手术组肾小管凋亡细胞数明显增多,Bcl-2、Bax表达均增强,Bax/Bcl-2比值增高;银杏叶提取物处理组较缺血再灌注组凋亡细胞数明显减少,Bcl-2表达进一步增强,而Bax表达减弱,Bax/Bcl-2降低。结论银杏叶提取物可能通过影响Bcl-2、Bax表达,抑制细胞凋亡,从而发挥对缺血再灌注损伤肾脏的保护作用。
Objective To study the effects of Ginkgo biloba extract （EGb） on the expression of Bcl- 2 and Bax genes during renal ischemia - reperfusion （I/R） in rats. Methods The model of bilateral post - ischemic renal injury was set up by clamping renal pedicles for 45 minutes and allowing repeffusion for 24 hours. After that, the apoptosis of tubular cells was assessed by terminal - deoxynucleofidyl transferase mediated d- UTP nick end labeling （TUNEL） method, the expression of Bcl - 2 and Bax was examined by immunohistochemistry. Results Compared with the results of sham operation group, significant increase of TUNEL positive cells, higher expression of Bax and Bcl - 2, and increased Bax/Bcl - 2 ratio were observed in I/R group （ P 〈 0.01 ）. The EGb pretreatment group showed a significantly lower number of apoptotic positive nuclei than the I/R group （P 〈 0.01 ）, along with increased expression of Bcl- 2, decreased expression of Bax and decreased Bax/Bcl- 2 ratio. Conclusion Inhibition of apoptosis and regulation of Bcl - 2/Bax expression may be responsible for the renal protective effects of EGb in the rats undergoing renal ischemia- reperfusion.
出处
《徐州医学院学报》
CAS
2006年第5期394-397,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省徐州市科技基金资助项目(20022037)
关键词
银杏叶提取物
缺血-再灌注损伤
凋亡
基因表达
Ginkgo biloba extract
ischemia- reperfusion injury
apoptosis
gene expression
