摘要
建立了柱前衍生化、高效液相色谱法（HPLC）测定化妆品中甲醛的方法。样品用乙腈溶解，加水稀释后于旋盖离心管中振荡提取2h，离心后分取部分提取液与2，4-二硝基苯肼在弱酸条件下衍生反应，衍生物直接HPLC测定。以乙腈-水（65＋35）为流动相，流速为1．0mL·min^-1，Supelco Discovery C18柱（4．6min×250mm，5μm）为分离柱，365nm紫外波长下检测，在5．0，50．0及100．0μg·g^-1甲醛的浓度水平作了精密度及回收率试验，回收率为70％～102％，相对标准偏差〈5．0％，检出限为5．0μg·g^-1。
HPLC was applied to the determination of formaldehyde in cosmetics. Formaldehyde in cosmetic sample was extracted with acetonitrile. An aliquot of the extract was taken and derivatized with 2, 4- dinitrophenylhydrazine in a weakly acidic medium. The derivatized product was determined by HPLC. The Supelco Discovery C18 column （4. 6 mm× 250 mm, 5μm） was used in the analysis. The mobile phase used was a mixture of acetonitrile and water with a ratio of 65 to 35 by volume. The flow-rate chosen was 1.0 mL . min^-1. U-V-detection was made at the wavelength of 365 nm Precision and recovery tests were made at the concentration level of 5. 0, 50. 0 and 100. 0 μg . g^-1 of formaldehyde, and the results obtained were as follows： recovery in the range of 70%- 102%, RSD〈5. 0%, detection limit 5.0μg . g^-1.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2006年第9期723-725,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
