摘要
目的:观察从黄蜀葵花中提取的有效成分—黄蜀葵花总黄酮对大鼠佐剂性关节炎的防治作用,并分析其作用途径。方法:实验于2004-03/07在安徽医科大学基础医学院药理教研室完成。选择SD雄性大鼠60只,按随机数字表法分为6组,即正常对照组、佐剂性关节炎模型组、黄蜀葵花总黄酮25,50,100mg/kg组(黄蜀葵花总黄酮由安徽省医学研究所提供,为棕黄色粉末)和雷公藤多苷40mg/kg组,每组10只。除正常对照组外,各组大鼠均于左后足趾皮内注射0.1mL弗氏完全佐剂诱发大鼠佐剂性关节炎模型,足容积法测原发侧及继发侧足肿胀度(致炎后容积-致炎前容积),并进行多发性关节炎评分(5级评分:0分无红肿;4分包括踝关节在内的全部足爪红肿。根据未注射佐剂的其余3个肢体的病变程度累计积分,最高为12分)。应用苏木精-伊红染色检测非致炎侧踝关节的病理改变;MTT法检测脾淋巴细胞增殖反应以及大鼠腹腔巨噬细胞产生白细胞介素1水平,放射免疫法测定腹腔巨噬细胞产生肿瘤坏死因子α和前列腺素E2含量,硝酸还原酶法测定血清中一氧化氮含量。结果:60只大鼠全部进入结果分析,无脱失。①各组大鼠原发性足肿胀度的比较:佐剂性关节炎模型组大鼠致炎后不同时段足肿胀度均显著高于正常对照组(P<0.01),黄蜀葵花总黄酮25,50,100mg/kg组大鼠的在不同时段的原发性足肿胀度均显著低于模型组(P<0.05～0.01)。②各组大鼠继发性足肿胀度及多发性关节炎指数比较:黄蜀葵花总黄酮25,50,100mg/kg组大鼠的继发性足肿胀度和多发性关节炎评分均显著低于佐剂性关节炎模型组(P<0.05～0.01),以黄蜀葵花总黄酮100mg/kg组作用最为显著。③各组大鼠非致炎侧踝关节病理改变比较:不同剂量的黄蜀葵花总黄酮对佐剂性关节炎大鼠关节组织的上述病理改变有不同程度的改善作用,黄蜀葵花总黄酮100mg/kg组作用最明显。④各组大鼠的脾细胞增殖情况比较:黄蜀葵花总黄酮50,100mg/kg组可明显促进佐剂性关节炎大鼠低下的脾淋巴细胞增殖反应(P<0.05)。⑤各组大鼠腹腔巨噬细胞产生的白细胞介素1、前列腺素E2、肿瘤坏死因子α和血清一氧化氮含量比较:佐剂性关节炎模型组大鼠腹腔巨噬细胞产生的白细胞介素1、前列腺素E2、肿瘤坏死因子α及血清一氧化氮含量均显著高于正常对照组(P<0.01);黄蜀葵花总黄酮各剂量组均显著低于模型组(P<0.05～0.01)。结论:黄蜀葵花总黄酮各剂量组能减轻佐剂性关节炎大鼠的原发性、继发性关节肿胀和多发性关节炎程度,以黄蜀葵花总黄酮100mg/kg组作用最为显著,其作用途径可能与其调节机体的异常免疫有关。
AIM： To investigate the preventive and therapeutic effects of total flavone of abelmoschus manihot L medic （TFA） on adjuvant arthritis （AA） in rats, and analyze its mechanism. METHODS： The experiment was carried out in the Staff Room of Pharmacology, School of Basic Medicine, Anhui Medical University from March to July in 2004. Sixty SD male rats were randomized into 6 groups： control group, AA model group, TFA groups of different dosages （25, 50, 100 mg/kg, TFA in brown yellow powder was offered by Anhui Institute of Medical Sciences） and tripterygium glycosides group, with 10 rats in each. The AA model was induced by injection of intradermal Freund＇ complete adjuvant （0.1 mL） into left hindpaw of rats except control group. Primary and secondary hindpaw swelling of AA rats were measured with volume meter （volume after inflammation-volume before inflammation）, and multiple arthritis indexes were scored by 5 grades. （0 point： No red swelling was found; 4 points： All the toes and ankle joints were swelled. The scores were accumulated with the lesions in other 3 uninjected limbs, and total score was 12 points.） The pathological changes of inflammatory ankle joint were observed by hematoxylin-eosin staining. The splenetic lymphocyte proliferation was examined by MTT assay and the activities of interleukin-1 （IL-1） from peritoneal macrophages were examined by thymocyte proliferation method. Tumor necrosis factor alpha （TNF-α） and prostaglandin （PG） E2 production were determined by radioimmunoassay, and NO levels in serum were determined by nitrate reductase assay. RESULTS： Totally 60 rats were involved in the result analysis without loss.①Comparisons of primary swelling： The primary swellings at different time phases after acute inflammation were significantly higher in AA model group than those of control group （P 〈 0.01）, and significantly higher in model group than TFA groups （P 〈 0.05-0.01）.②Comparisons of secondary swelling and multiple arthritis index： The scores of secondary inflammatory swelling and multiple arthritis index were notably decreased in TFA groups compared with those in AA model group （P 〈 0.05-0.01）, especially 100 mg/kg TFA inhibited them most outstandingly. ③ Comparisons of pathological changes of ankle joint without acute inflammation： Different dosages of TFA improved above pathological changes of AA rats to different extents, and 100 mg/kg TFA played the most obvious rule.④Comparisons of splenetic proliferation： TFA of 50 and 100 mg/kg increased splenetic lymphocyte proliferative reaction of AA rats obviously.⑤Comparisons of various levels： The productions of IL-1,TNF-α and PGE2 from peritoneal macrophages and NO levels in serum were remarkably higher in AA model group than in control group （P 〈 0.01）, but remarkably lower in TFA groups than in model group （P 〈 0.05-0.01）. CONCLUSION： TFA, especially at the dose of 100 mg/kg, can reduce the primary and secondary swelling as well as multiple arthritis in AA rats, and the mechanism may be related to its immunoregulative function.
出处
《中国临床康复》
CSCD
北大核心
2006年第35期34-37,共4页
Chinese Journal of Clinical Rehabilitation
