摘要
目的探讨凝血酶对大鼠脑内MMP-9、MMP-2表达的影响。方法Wistar大鼠随机分为假手术组及实验组。实验组脑内注入凝血酶,在不同时间点采用干湿重法测脑水含量,免疫组化方法检测脑内MMP-9、MMP-2的表达。假手术组注入等量生理盐水。结果脑组织水含量在凝血酶注入后6h开始增加,3d达高峰。MMP-9阳性细胞在注射凝血酶后6h即开始表达增加,与对照组相比差异显著(P<0.01),3d达高峰,随后持续下降。阳性微血管数也有相似的变化趋势。MMP-2阳性细胞在注射凝血酶后1d才开始有少量表达,后持续增多,5d达高峰,随后有所下降,但14d仍有明显表达,与对照组相比差异显著(P<0.01)。阳性微血管数也有相似的变化趋势。结论凝血酶在脑出血后脑水肿及脑组织损伤中起了关键的作用,MMP-9、MMP-2参与了急性期脑水肿、炎症反应等过程,MMP-2在损伤修复中可能有重要作用。
Objective To study expression of MMP-9 and MMP-2 in rats brain after injection thrombin. Methods Wistar rats were divided randomly into 2 groups,sham operated group and experiment group. The animals of experiment group were injected thrombin in brain. At the due time some rats were killed and brain tissue was removed to measure the water content by dry-wet weight method. The others were immunohistochemically stained to measure of the expression of MMP-9 and MMP-2. The animals of sham-operated group were injected normal saline. Results The water content of brain increased at 6h after injection thrombin,and peaked at 3d. Compared with sham-operated group,in experiment group the expression of MMP-9 positive cells were observed at 6h after injection thrombin （P〈0.01）,and peaked at 3d ,then decreased gradually. The number of MMP-9 positive micro-blood vessel had the same change tendency as the positive cells. A few of MMP-2 positive cells were observed until ld in experiment group.and peaked at 5 days,then decreased gradually. The increasing tendency lasted to 14d. The number of positive cells at 14d was significantly more than sham group （P〈0.01）. This tendency was similar to the number of MMP-2 positive micro-blood vessel. Conclusion Thrombin plays an important role in intracerebral hemorrhage on cerebral edema and tissue impairment. MMP-9 and MMP-2 contribute to brain edema and inflammatory reaction in the acute phase of ICH ,and MMP-2 is perhaps important in rehabilitation of the brain injury.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2006年第1期75-77,I0001,共4页
Journal of Apoplexy and Nervous Diseases
