摘要
用ＰＣＲ的方法，扩增了伪狂犬病病毒（ＰＲＶ）ｇｐ５０基因的一段较为保守区（２１６ｂｐ）．检测了不同ＰＲＶ毒株感染的ＢＨＫ细胞以及接种的家兔，并对ＰＣＲ检测的灵敏性作了初步研究，结果表明，ＰＣＲ法扩增ｇｐ５０基因具有较高特异性和灵敏性。
In this paper,a conservative fragment(216 bp) in gp50 gene of pseudorabies virus(PRV) was amplified by PCR technique.BHK 21 cells infected by different kinds of PRV strains and inoculated rabbits were detected.The sensitivity of PCR detection was also studied.The results demonstrated that PCR technique was quite special and sensitive to amplify gp50 gene and it was an effective method for detection PRV.
出处
《武汉大学学报（自然科学版）》
CSCD
1996年第6期787-790,共4页
Journal of Wuhan University(Natural Science Edition)
