摘要
以叶片为外植体，探讨红叶石楠红唇愈伤组织诱导及植株再生的方法。结果表明，无菌繁殖体系以MS＋BA0.3mg／L＋NAA1．5mg／L为佳；丛生芽的增殖以MS＋BA2．0mg／L＋IBA0．1mg／L＋GA33．0mg/L＋糖30g最适；生根培养用1／2MS＋IBA0．3mg／L较好。经生根的试管苗移栽于草炭：蛭石：珍珠岩：2：2：1的混合基质中，温度22～28％，相对湿度90％-100％，保湿7～10d，其间适当遮荫，25d的成活率超过95％。
The leaves of Photinafrasery cv. Red Tip were used as explants in tissue culture to induct callus and plantlet regeneration. The study results showed that the optimum medium was MS ＋ 0.3 mg BA/L ＋ 1.5 mg NAA/L for the callus induction;the optimum medium was MS ＋ 2.0 BA mg/L,0.1 mg IBA/L ＋ 3.0 mg GA3/L ＋ 30g sugar/L for proliferation of the sub-cultured buds; the optimum medium was 1/2 MS ＋ 0.3 mg IBA/L for rooting. The survival rates of the rooting planflets transplanted into the substrate containing 40% venniculate,40% pearlite, and 20% peat （v/v） was 95% after 25 days transplanting under the conditions of 22 - 28℃, 90%-100% of relative humidity, and keeping moisture for 7-10 d.
出处
《浙江农业学报》
CSCD
2006年第4期264-267,共4页
Acta Agriculturae Zhejiangensis
关键词
红叶石楠
愈伤组织
诱导
植株再生
Photina frasery
callus
induction
plantlet regeneration
