摘要
探讨并构建了三酶复合法提取大豆多肽的新工艺。即将大豆分离蛋白经三酶复合酶解、活性炭脱色、超滤、真空浓缩和离心喷雾干燥等提取工艺,最终获得大豆多肽。其制备的关键工艺条件为①最佳复合酶解条件:中性蛋白酶、木瓜蛋白酶、菠萝蛋白酶比例为1∶2∶4,pH 7.0,底物浓度4.0%,酶解时间9 h;②最佳脱色处理条件:粉末活性炭用量2.0%,温度50℃,pH 3.0,吸附时间3 h;③超滤分离条件:NF-1纳滤膜,压力1.0 MPa,膜通量26.0 L/(m2.h)。
The new extraction technique of soybean pepfides by triple-enzyme composite hydrolysis was established in this study. Soybean peptides were obtained from isolated soybean protein by triple-enzyme composite hydrolysis and purification. The result showed that the best conditions of composite enzymatic hydrolysis were： the composite enzymes were made up of neutral proteinase, papain and bromelain with the ratio of 1：2：4,pH 7.0,4.0% of substrate concentration, and hydrolysis time for 9 h; the best conditions of decoloration were： 2.0% of activated carbon, 50℃ of temperature, pH 3.0, adsorption time for 3 h; the ultra-filtration conditions were： NF-1 nanometer membrane, 1.0 MPa of pressure and 26.0 L/（m^2·h） of the permeate flux.
出处
《浙江农业学报》
CSCD
2006年第4期241-245,共5页
Acta Agriculturae Zhejiangensis
基金
浙江省科技计划项目(2003C32022)
关键词
大豆多肽
复合酶解
脱色
超滤
soybean peptides
composite enzymatic hydrolysis
decoloration
uhra-filtration
