摘要
建立了玉米秸秆采用蒸汽爆破预处理、纤维素酶水解和戊糖己糖同步发酵技术制取酒精的中间试验装置。玉米秸秆在1.6～2.0 MPa条件下蒸汽爆破预处理,在提高玉米秸秆对纤维素酶可及度的同时,玉米秸秆中纤维素、木聚糖和木质素损失分别为4.08%、40.02%和9.91%。里氏木霉以10%的原料制备纤维素酶,并用于降解剩余的90%的原料,滤纸酶活力和纤维素酶水解得率分别为2.27 FPIU/mL和71.3%。初始还原物浓度为43.65 g/L的水解糖液经树干毕赤酵母发酵16 h,还原物利用率和酒精得率分别为87.17%和0.43 g/g(酒精/消耗的糖)。
A pilot system including steam explosion pretreatment, cellulose enzymatic hydrolysis and pentose-hexose simultaneously fermentation was integrated. The cellulose accessibility to cellulase was improved when the corn stalk was pretreated under 1.6-2.0 MPa for 10-15 min, meanwhile the loss of cellulose, xylan and lignin were 4.08%, 40.02% and 9.91%, respectively. 10% of raw materials were used for cellulase preparation by Trichoderma reesei and was used to hydrolyze the other 90% raw materials in pilot system, the filter paper activity and cellulose hydrolysis yield were 2.27 FPIU/mL and 71.3%, respectively. The hydrolysate containing 43.65 g/L reducing sugars was fermented by Pichia stipitis for 16 h, the consumption of reducing substances and ethanol yield were 87.17% and 0.43 g/g（ethanol/sugar consumption）.
出处
《纤维素科学与技术》
CAS
CSCD
2006年第3期37-40,70,共5页
Journal of Cellulose Science and Technology
基金
国家自然科学基金资助项目(30471361)
江苏省高技术计划项目(BG2005327)
关键词
酒精
蒸汽爆破
纤维素酶
戊糖发酵
ethanol
steam explosion
ceUulase
pentose fermentation
