摘要
目的了解铜绿假单胞菌对氨基糖甙类抗生素的耐药状况,探讨其耐药机制。方法采用微量稀释法和纸片扩散法进行抗生素敏感试验,聚合酶链反应(PCR)检测5种氨基糖甙类修饰酶耐药基因。结果临床分离的264株铜绿假单胞菌对庆大霉素、妥布霉素和阿米卡星的耐药率分别为8.6%、7.5%和2.9%。被检耐药基因的20株铜绿假单胞菌中有6株检出氨基糖甙类修饰酶耐药基因,分别是aac(3)-Ⅱ(2/20)、aac(6′)-Ⅰ、(1/20)、ant(3")-Ⅰ(1/20)和ant(2")-Ⅱ(2/20)。结论该院铜绿假单胞菌对常用氨基糖甙类抗生素的敏感性高,此类抗生素仍是临床治疗铜绿假单胞菌感染的优先选择。产生氨基糖甙类修饰酶是该院临床感染铜绿假单胞菌对氨基糖甙类抗生素的耐药机制之一。
Objective To investigate aminoglycosides resistance condition and its mechanism. Methods Antibiotics sensitivity tests were taken by microdilute method, and five genes of aminoglycoside-modifying enzymes were detected and sequenced by PCR. Results The resistant rates of the 264 strains pseudomonas aeruginosa to gentamyci,tobramycin and amikacin were 8.6%, 7.5%, 2.9% respectively. In 6 of 20 strains of clinically infective pseudomonas aeruginosa, aminoglycoside-modifying enzymes genes that were respectively aac（3）-Ⅱ（2/20）, aac（6＇ ）- Ⅰ （1/20）, ant（3＂）- Ⅰ （1/20） and ant （2＂）-Ⅱ （2/20） were detected. Conclusion The rate of sensitivity to aminoglycosides in pseudomonas aeruginosa isolated from our hospital is high. These antibiotics remain priority choice in clinical treatment in pseudomonas aeruginosa infections. Amimoglycoside-modifying enzymes genes of pseudomonas aeruginosa might be one of mechanisms of resistance to aminoglycosides.
出处
《国际检验医学杂志》
CAS
2006年第8期677-678,690,共3页
International Journal of Laboratory Medicine
