摘要
目的观察肢体缺血-再灌流时心功能的变化和心肌细胞凋亡状况。方法健康家兔30只,随机均分为假手术对照组(SC)、单纯缺血组(Ⅰ)和缺血再灌流组(JR)。免疫组化法检测心肌组织Bcl-2和Bax蛋白表达；原位缺口末端标记法(TUNEL)检测心肌细胞凋亡；比色法测定心肌组织超氧化物岐化酶(SOD)和髓过氧化物酶(MPO)活性-丙二醛(MDA)含量,测定血清乳酸脱氢酶(LDH)活性；通过生理记录仪观察心功能指标；光镜下观察心肌病变。结果IR组心肌组织细胞凋亡指数明显高于其它两组,Bax蛋白表达明显增多,同时IR组心肌组织SOD活性降低,MDA含量增高,血清LDH活性增高,左心室功能下降,且MDA含量与LDH活性、Bax蛋白表达和凋亡指数之间呈显著正相关,光镜下可见心肌病变。结论肢体缺血-再灌流可造成心肌损伤,心功能下降,与氧化损伤和细胞凋亡有关。
Objective To investigate the changes of cardiac function and myocardial apoptosis after limb ischemia reperfusion in rabbits. Methods Thirty rabbits were randomly divided into 3 groups： sham-operated control group （group SC）, ischemia group （group I, 8 hours of bilateral hind limb ischemia） and ischemia reperfusion group （group IR, 4 hours of bilateral hind limb ischemia and 4 hours of reperfusion） . Immunohistochemical studies and TUNEL were done to evaluate cell apoptosis. The SOD activity and MDA content in myocardial tissue and plasma LDH activity were determined. The left ventricular function were observed by physiology recording instrument. The cardiac histopathologic changes in experimental rabbits were observed. Results In group IR, the apoptosis index and expression of Bax were significantly increased compared with the other two groups. Similarly, ischemia reperfusion reduced SOD activity and enhanced LDH activity and MDA level, and the left ventricular function decreased in group IR. There was a positive correlation among the MDA content and the LDH activity, the expression of bax, the number of apoptosis cells. Under light microscope, myocardial impairment was found in group IR. Conclusion The myocardial injury occured and the left ventricular function decreased after limb ischemia reperfusion, and they were associated with oxidative damage and apoptosis.
出处
《中华急诊医学杂志》
CAS
CSCD
2006年第8期696-699,共4页
Chinese Journal of Emergency Medicine
基金
浙江省中医药科研基金项目(2004C124)
温州市科技局科研基金项目(Y2003A112)
关键词
再灌流损伤
肢体
凋亡
心功能
Reperfusion injury
Limb
Apoptosis
Cardiac function
