摘要
基于在碱性介质中,白藜芦醇对I-增强N-氯代丁二酰亚胺-鲁米诺化学发光体系的阻抑作用,建立了测定白藜芦醇的流动注射化学发光分析新方法。优化了流动注射化学发光的分析条件和探讨了反应的可能机理。测定白藜芦醇的线性响应质量浓度范围为2.5×10-9~8.0×10-8g.mL-1,检出限为6.3×10-10g.mL-1,用5.0×10-8g.mL-1的白藜芦醇标准溶液进行11次测定,相对标准偏差(RSD)为2.2%。应用本法测定了虎杖、葡萄酒、葡萄皮样品中的白藜芦醇含量,结果满意。
A novel flow injection chemiluminescence method has been developed for the determination of resveratrol based on its inhibition of the chemiluminescence from the iodide - N-chlororsuccinimide - luminol system in alkaline medium. The possible mechanism of the chemiluminesce reaction and the optimum analytical operating conditions have been studied. The linear range of resveratrol determination is 2.5×10^-9～8.0×10^-8g·mL^-1 with a detection limit of 6.3×10^10g·mL^-1. The relative standard deviation（RSD） （n = 11 ） is 2.2% for 5.0×10～g·mL^-1 resveratrol. The method has been applied to determine the content of resveratrol in grape skin, grape wine and traditional Chinese medicine polygonumcuspidatum seib with satisfactory results.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第4期88-91,共4页
Journal of Instrumental Analysis
基金
山东省中青年学术骨干与学科带头人培养基金资助
