摘要
目的研究二氯乙酸(DCA)和三氯乙酸(TCA)的DNA损伤效应。方法分别以10、20、40μg/mlDCA和10、20、40、80μg/mlTCA染毒V79细胞和昆明种小鼠肝原代细胞,染毒1h后进行彗星试验。电泳结束后,溴化乙啶(EB)染色,在荧光显微镜下观察、计数拖尾细胞的尾长。结果DCA在10～40μg/ml、TCA在10～80μg/ml时,各试验组V79细胞的平均尾长均大于阴性对照组尾长,差异均有统计学意义(均P<0.01)。DCA为20～80μg/ml、TCA为10～80μg/ml时,各试验组肝原代细胞的平均尾长均大于阴性对照组尾长,差异均有统计学意义(P<0.01)。结论DCA和TCA可以造成哺乳动物细胞DNA链断裂损伤,其致癌作用可能通过损伤DNA引发,均可能属遗传毒性致癌物。
Objective To study the DNA damage induced by dichloroacetic acid（DCA） and trichloroacetic acid（TCA） which are from drinking water disinfection by-products. Methods V79 cells and hepatocytes of mice were treated with DCA and TCA for 1 hour and then were tested by comet assay. After stained by EB, tail length of the cells were observed and counted under the fluorescence microscope. Results Both DCA and TCA could result in the increasing of average tail length of the treated cells whether they were V79 cells or hepatoeytes of mice and the dose-response relationships were seen. Conclusion Both DCA and TCA can cause DNA damage of mammalian cells and this may be related to the carcinogenic mechanism. DCA and TCA belong to genetic carcinogens.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第4期294-296,共3页
Journal of Environment and Health
基金
国家自然科学基金资助项目(39670625)
关键词
水
消毒
DNA损伤
消毒副产物
二氯乙酸
三氯乙酸
彗星试验
Water
, Disinfection
DNA damage
Disinfection by-products
Dichloroacetic acid
Trichloroacetic acid
Comet assay
