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特异性中和抗体终止猪体内猪繁殖与呼吸综合征病毒(PRRSV)复制 被引量：7

Specific Neutralizing Antibodies Terminate Replication of Porcine Reproductive and Respiratory Syndrome Virus(PRRSV) in PRRSV Infected Pigs
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摘要 根据猪繁殖与呼吸综合征病毒(PRRSV)美洲型标准株(VR-2332)的ORF 6及ORF 7部分保守序列,设计合成1对特异引物,通过优化RT-PCR反应条件,建立了从血清中检测PRRSV RNA的RT-PCR诊断方法。60日龄仔猪经鼻腔接种1 mL 105.6TC ID50/mL PRRSV强毒液,7 d后肌肉注射中和效价为1∶64的特异性高免血清,每隔1周利用RT-PCR检测猪体内PRRSV RNA,5周内PRRSV RNA检测均为阴性,表明高滴度特异性中和抗体完全终止了PRRSV感染猪体内的病毒复制。 A set of two oligonucliotide primers were designed according to the reserved sequences of ORF6 and ORF7 of porcine reproductive and respiratory syndrome virus（PRRSV） strain VR-2322. A diagnostic RT-PCR assay has been developed against PRRSV RNA through optimizing the reaction condition. Pigs were challenged intranasally on 0 day old with 1 mL of PRRSV virulent strain containing 10^5.6 TCID50/mL. 7 days after being challenged pigs were injected by specific serum of neutralizing titer of 1 ： 64. Serum was collected at weekly internal from 0 to 42 days post-infection and RT-PCR assay was applied for detection of PRRSV RNA in serum. The results showed that high titer neutralizing antibody could terminat the replication of PRRSV in infected porcines completely.
作者 夏平安 崔保安 张红英 梁宏德 杨霞 陈丽颖
机构地区 河南农业大学牧医工程学院
出处 《中国兽医学报》 CAS CSCD 北大核心 2006年第4期354-356,共3页 Chinese Journal of Veterinary Science
关键词 PRRSV 中和抗体 RT—PCR 猪 繁殖与呼吸综合征病毒 PRRSV neutralizing antibody RT-PCR
分类号 S852.65 [农业科学—基础兽医学] S858.28 [农业科学—临床兽医学]
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参考文献13

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同被引文献50

  • 1郭宝清,陈章水,刘文兴,崔益洙.从疑似PRRS流产胎儿分离PRRSV的研究[J].中国畜禽传染病,1996(2):1-5. 被引量：935
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引证文献7

二级引证文献15

中国兽医学报
2006年 第4期

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