摘要
用柱层析法在氯仿和甲醇液中对白头翁皂苷酶解产物进行分离纯化,得到1种单糖基皂苷和皂苷元。经薄层层析和高效液相色谱检测确定:单糖基皂苷为3-O-α-L-阿拉伯糖-3β,23-二烃基-Δ20(29)-羽扇豆烯-28-酸,其得率为5.1%。高效液相色谱检测结果显示所得纯品纯度可达95%以上。
Monosaccharide Pulsatilla saponin product and aglycone from enzyme reaction are separated and purified by silica gel column gradient elution with the solvent of chloroform and methanol. The monosaccharide Pulsatilla saponin （3-O-α-L-ara-313,23-dihydroxylup-20 （29）-en-28-oic acid） and aglycone are obtained in the product from enzyme reaction by TLC and HPLC. The monosaccharide Pul- satilla saponin yield is 5.1% with a purity over 95%.
出处
《大连轻工业学院学报》
2006年第2期79-82,共4页
Journal of Dalian Institute of Light Industry
基金
国家自然科学基金资助项目(303717442047601730470055)
关键词
单糖基白头翁皂苷
分离
纯化
Monosaccharide Pulsatilla saponin separation purification
