摘要
目的原代分离、纯化、培养大鼠肌卫星细胞,观察体外培养肌卫星细胞的增殖与分化特性及对类胰岛素样生长因子Ⅰ(IGF-Ⅰ)刺激的反应。方法选用Wistar大鼠,切取双下肢及背部肌肉,两步酶消化法分离卫星细胞,不连续密度梯度离心法纯化后体外培养,观察其生长与分化特性,绘制生长曲线及融合率曲线,MTT法测定卫星细胞对不同浓度IGF-Ⅰ刺激的反应,并对其生长分数及融合率给予观察。结果此方法可以获得足量、纯度较高的肌卫星细胞。结论适宜浓度(100ng/ml)的IGF-Ⅰ对肌卫星细胞有较强的促增殖及促分化作用。
Objective To observe the proliferation and differentiation properties of rat skeletal muscle satellite cells that are dissected, cultured and purified in vitro and investigate their response to stimulation by insulin-like growth factor Ⅰ （IGF-Ⅰ）. Methods The cells were isolated from Wistar rats by two-step enzyme digestion and purified by the Percoll density centrifugation to observe their proliferation and differentiation properties and draw growth curve and fusion rate curve. Then, the response of the satellite cells to stimulation by IGF-Ⅰ at various concentrations was determined by using MTT method. Results Plenty and purified satellite cells were obtained via these methods. Conclusion IGF-Ⅰ at appropriate concentration （100 ng/ml） can promote the proliferation and differentiation of rat skeletal muscle satellite cells.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2006年第7期531-534,共4页
Chinese Journal of Trauma
关键词
卫星细胞
胰岛素样生长因子Ⅰ
细胞培养
肌萎缩
Satellite cells
Insulin-like growth factor
Cell cultivation
Muscular atrophy
