摘要
本文对ISSR-PCR扩增苦瓜基因组DNA的主要影响因子进行了筛选和分析。试验结果表明，25μl的反应体系中采用20～30ng的模板DNA、1μmol/L ISSR引物、1U Tag DNA聚合酶，以及48℃～52℃的复性温度为苦瓜ISSR-PCR扩增条件的最佳选择。苦瓜ISSR-PCR扩增条件的优化为进行苦瓜种群间遗传分化的研究奠定了基础。
The factors influencing ISSR-PCR experiments to analyze the genetic divergence in bitter melon （Momoradica charantia L. ） were investigated. Comparative experiments on the concentrations of template DNA, ISSR primer, Taq DNA polymerase and the annealing temperature were carried out. The results showed that the optimum reaction condition of ISSR experiment was 20～30ng DNA template, 1 μmol/L ISSR primer, 1U Tag DNA polymerase and annealing temperature of 48 ℃～52℃ in each reaction system of 25μl, which set up a basis for the study of genetic divergence among bitter melon populations.
出处
《核农学报》
CAS
CSCD
北大核心
2006年第3期215-217,168,共4页
Journal of Nuclear Agricultural Sciences
关键词
苦瓜
ISSR
影响因子
PCR
反应体系
Momoradica charantia L.
ISSR
influencing factors
PCR
reaction system
