摘要
目的:建立阿尔茨海默病(AD)的大鼠模型,并观察远志皂苷对AD模型大鼠β-淀粉样蛋白(Aβ)沉积及其神经细胞毒性的影响。方法:在大鼠右侧基底核区定向注射凝聚态Aβ1-40和鹅膏蕈氨酸建立AD模型,20只AD大鼠分为模型组和远志皂苷组,相同部位注射生理盐水作为假手术组。30天后,采用HE、N issl、透射电子显微镜和免疫组化染色等方法观察海马区神经细胞形态和基底核Aβ沉积变化。结果:脑内Aβ注射后,模型大鼠海马区神经元数目明显比假手术组减少,基底核M eynert细胞区可见棕黄色斑块沉积。电镜下可见神经元内脂褐质含量增加,线粒体等细胞器出现明显变性改变。结论:凝聚态Aβ1-40基底核定位注射具有明显的在体神经毒性作用,可较好地模拟AD病理表现,远志皂苷对Aβ引起的神经细胞凋亡有明显的保护作用。
Objective： To establish an animal model of Alzheimer＇ s disease（AD） and to observe the therapeutic effect of tenuigenin（TEN） on deposition of β-amyloid protein（Aβ） and its neurotoxicity. Methods： A combined injection of aggregated Aβ1-40 and ibotenic acid（IBO） into the right nucleus basalis magnocellularis （NBM） was used to produce the AD rats model. The rats were divided into two group： model group and medicine group （ made with feeding the model group with TEN for 30 days） , the control group were injected with sterile normal saline in the same way as the above for the sham operation group. At 30 clays after operation, the brain tissues were stained by HE staining, Nissl staining and cell ultrastructure were examined by electron microscope observation and deposition of Aβ in the NBM were examined by immunohistochemical technique. Results： The number of neurons in the model rats were less than the sham operation group, the deposition of Immunohistochemical study demonstrated that the injected Aβ1-40 in NBM was increased obviously and it is obvious that brown yellow macule deposition, the lipofuscin increased, obviously and the organelle such as mitochondria, RER and nuclear envelope were deformed in neuron. Conclusions：These results suggest that the aggregated Aβ deposition in the NBM related to the neuronal degeneration in the hippoeampus which showed similar pathological characterizations of AD, tenuigenin can reduce the neuronal degeneration and the area of the remained Aβ of AD rats and could protect neuron apoptosis caused by toxicity of Aβ.
出处
《激光生物学报》
CAS
CSCD
2006年第3期294-298,共5页
Acta Laser Biology Sinica
基金
安徽省教育厅自然科学基金(KJ028)
