摘要
目的:探索小鼠骨髓干细胞,在同种异体不同途径移植后向受体肝脏迁移的情况．方法:以红色荧光染料PKH26标记从小鼠骨髓中分离出的干细胞,分别从小鼠的尾静脉和门静脉注入同种异体的CCL4-AAF造成肝损伤的小鼠体内,移植2 wk后分别取肝组织, 通过荧光显微镜观察两种移植途径对小鼠骨髓干细胞向肝脏迁移的影响．结果:两组鼠的肝小叶中央静脉及汇管区均可见新生的肝细胞,PKH26标记阳性的细胞在门静脉移植组20倍镜下每张切片平均为175．4 个,在尾静脉移植组平均172．9个(P>0．05)．结论:骨髓干细胞向肝脏迁移的细胞数与移植途径无关．
AIM： To explore the amount of bone marrow mononuclear cells migrating to the liver during allografting in mice.
METHODS： Bone marrow stem cells were isolated from the bone marrow of mice by lymphocyte separation medium and labeled with PKH26. The labeled cells were subsequently infused into the caudal or portal veins of different mice with homogeneity, in which hepatic injury was induced by carbon tetrachloride and 2-acetylminofluorene. Two weeks after transplantation, the migration status of the cells was studied by fluorescence microscopy. RESULTS： Liver cells were found at portal region and central veins of hepatic lobules in all the mice. The PKH26 labeled cells appeared at an average frequency of 175.4 per section from the livers of mice received transplantation via portal veins, and at an average frequency of 172.9 per section from the livers of mice underwent transplantation via caudal veins. There was no significant difference between them （P 〉 0.05）.
CONCLUSION： Bone marrow stem cells are able to migrate to liver, the numbers of which have no correlation with the approach of transplantation.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第14期1408-1411,共4页
World Chinese Journal of Digestology
基金
黑龙江省自然科学基金项目
No.D200536~~
关键词
骨髓干细胞
移植
静脉
Bone marrow stem cell
Transplanta- tion
Vein
