摘要
为获得白皮大蒜组培繁殖体系,以其茎尖为材料,通过BA和NAA不同激素配比试验研究,结果表明:诱导愈伤组织的最适培养基为:MS+BA0.2mg/L+NAA0.5mg/L,诱导率达60%;诱导不定芽的最适培养基为:MS+BA1.0mg/L+NAA0.1mg/L,丛生芽繁殖系数高达80%。同时,为建立扩繁最佳体系,分别研究了激素、pH、谷氨酸钠三因素对不定芽增殖系数的调节效果,研究表明:添加0.5mg/L的GA3使增值系数提高到13.8;pH5.8最利于不定芽增值;添加10mg/L谷氨酸钠能在多次继代培养中保持较高的增值系数。
The experiments about tissue culture of Garlic shoot-tip were made in MS mediums supplemented with different phytohormones. And then the best medium for rapid propagation of Garlic was obtained. The results were as follows： the optimized medium for inducing callus was MS＋BA 0.2mg/L＋ NAA0.5mg/L, and the growth rate of callus was 60%. The suitable medium for inducing adventitious buds was MS ＋BA1.0 mg/L＋NAA 0.1mg/L, and the highest propagation rate reached 80%. At the same time, the effects of different phytohormones, pH and glutamate on the propagation coefficient in Garlic have been researched. Then the results indicated： Propagation coefficient is raised to 13.8 in the medium supplemented with 0.5mg/L GA3. And it also can be raised in pH5.8. Propagation coefficient in subculture is remained at high level in 10mg/L glutamate.
出处
《中国农学通报》
CSCD
2006年第6期224-226,共3页
Chinese Agricultural Science Bulletin
基金
重庆市农业局资助项目"蔬菜营养体快繁技术研究"。
关键词
大蒜
离体培养
茎尖培养
增殖系数
Garlic （Allium sativum L.）, In vitro culture, Shoot apex tissue culture, Propagation coefficient
